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Clinical Bacteriology > (M) Lec 6.1: Bacterial Identification > Flashcards

(M) Lec 6.1: Bacterial Identification Flashcards

1
Q

What are the different multitest systems?

A
  1. bioMerieux’s API systems
  2. BD BBL
  3. Remel’s RapID

+Biomic V3

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2
Q

This type of multitest system is usually used to identify gram negative and gram positive organisms.

A

bioMerieux’s API systems

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3
Q

Give the type of API system according to the time and organism given.

  • Organism/s Identified: Enterobacteriaceae and other non-fastidious gram negative organisms
  • Duration/Time: 18 to 24 hours
A

API 20E

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4
Q

Give the type of API system according to the time and organism given.

  • Organism/s Identified: Entereobacteriaceae
  • Duration/Time: 4 hours
A

API Rapid 20E

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5
Q

Give the type of API system according to the time and organism given.

  • Organism/s Identified: Gram negative non-Enterobacteriaceae
  • Duration/Time: 24 to 48 hours

Let me know if I can make this easier (kasi I don’t wanna put stuff here na di ko sure) pero if tama pagkakaintindi ko, it’s every other gram negative organism na hindi Enterobacteriaceae? Hehe, let me know if same tayo ng understanding mwa.

A

API 20NE

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6
Q

Give the type of API system according to the time and organism given.

  • Organism/s Identified: Neisseria, Haemophilus, Branhamella catarrhalis
  • Duration/Time: 2 hours
A

API NH

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7
Q

What type of API systems are used for gram negative organisms?

A
  • API 20E
  • API Rapid 20E
  • API 20NE
  • API NH
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8
Q

Give the type of API system according to the time and organism given.

  • Organism/s Identified: Clinical Staphylococci and Micrococci

Hehe, walang time e.

A

API Staph

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9
Q

Give the type of API system according to the time and organism given.

  • Organism/s Identified: Commonly occurring Staphylococci
  • Duration/Time: 2 hours
A

RAPIDEC

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10
Q

Give the type of API system according to the time and organism given.

  • Organism/s Identified: Streptococci and Enterococci
  • Duration/Time: 4 hours OR 24 hours
A

API 20 Strep

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11
Q

Give the type of API system according to the time and organism given.

  • Organism/s Identified: Corynebacteria and similar organisms
  • Duration/Time: 24 hours
A

API Coryne

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12
Q

Give the type of API system according to the time and organism given.

  • Organism/s Identified: Anaerobes
  • Duration/Time: 24 hours
A

API 20A

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13
Q

Give the type of API system according to the time and organism given.

  • Organism/s Identified: Anaerobes
  • Duration/Time: 4 hours
A

RAPID ID 32A

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14
Q

Give the type of API system according to the time and organism given.

  • Organism/s Identified: Yeast
  • Duration/Time: 48 to 72 hours
A

API 20C AUX

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15
Q

Which API systems are used for gram positive organisms?

A
  • API Staph
  • RAPIDEC
  • API 20 Strep
  • API Coryne
  • API 20A
  • Rapid ID 32A
  • API 20C AUX
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16
Q

These contain 15 standard biochemical tests in a compartmented tube to simultaneously inoculate 12 test media.

Agar substrate (tube) + long tube + system

A

BD BBL Enterotube II

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17
Q

This BD BBL system is used for the rapid identification of gram negative oxidative and fermentative bacilli

A

BD BBL Oxi/Ferm Tube II

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18
Q

This type of BD BBL can identify almost 500 organisms

A

BBL Crystal System

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19
Q

In this type of multitest system, there are panels for gram negative bacilli, enterics, and non-fermenters and for gram-positive aerobic organisms.

A

BD BBL

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20
Q

What are the different kinds of Remel’s RapID?

A
  • RapID One
  • RapID NF Plus
  • RapID CB Plus
  • RapID NH
  • RapID Staph Plus
  • RapID STR System
  • RapID Yeast Plus
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21
Q

Identify the RapID system based on the given information.

  • Has 19 substrates to identify
  • > 70 oxidase negative, gram negative bacilli (Enterobacteriaceae)
A

RapID One

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22
Q

Identify the RapID system based on the given information.

  • Oxidase positive enteric organisms
  • Non-fermenting gram negative bacilli
A

Rapid NF Plus

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23
Q

Identify the RapID system based on the given information.

  • Corynebacterium
  • Other gram positive Coryneform bacilli
A

RapID CB Plus

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24
Q

Identify the RapID system based on the given information.

  • Neisseria
  • Moraxella
  • Haemophilus and similar organisms
A

RapID NH

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25
Q

Identify the RapID system based on the given information.

  • 4 hour identification for yeast
A

RapID Yeast Plus

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25
Q

Identify the RapID system based on the given information.

  • Identification of 4 Staphylococci and related genera
A

RapID Staph Plus

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26
Q

Identify the RapID system based on the given information.

  • Identifies B hemolytic Streptococci and viridans Streptococci
A

RapID STR System

27
Q

It is a semiautomated procedure which involves inoculation, interpretation and reporting of results.

A

Biomic V3

28
Q

What are the three procedures involved in Biomic V3?

A
  1. Inoculation
  2. Interpretation
  3. Reporting of Results
29
Q

A system that involves color digital imaging

A

Biomic V3

30
Q

It is an open system that provides an instant digital record of all results and high-resolution images.

A

Biomic V3

31
Q

This semiautomated procedure can be interfaced with the lab information system.

A

Biomic V3

32
Q

What are the principles involved in the automated identification system?

A
  1. Colorimetry
  2. Nephelometry
  3. Fluorometry
33
Q

It is a color change in the pH indicator or other indicator as an organism metabolizes a particular substrate.

A

Spectrophotometry (Colorimetry)

34
Q
  • Light scattering - photometers + angles to the turbid suspension
  • Absorbance
A

Nephelometry

35
Q

Uses biochemical substrated that have a fluoroscent component

A

Fluoroscence

36
Q

It utilizes fluoroscence and detects change in pH.

A

Fluorometry

37
Q
  • Utilizes the principle of colorimetry
  • Uses 96 well microtiter trays of which 32 substrates can be used to identify Enterobacteriaceae
A

MicroScan System (Siemens Medical Solutions Diagnostics [FKA] Dade Behring)

38
Q
  • Uses gram positive panels, urinary tract panel, gram negative panels and anti-microbial susceptibility testing
A

MicroScan System (Siemens Medical Solutions Diagnostics [FKA] Dade Behring)

39
Q
  • Microtubes (D/F) + heavy suspension (15 to 18 hours at 35C)
  • The biochemical result is a 7 or 8 digit code number
  • Utilizes an automated tray
A

MicroScan System (Siemens Medical Solutions Diagnostics [FKA] Dade Behring)

40
Q

This piece of equipment in the MicroScan System detects bacterial growth or color changes as noted by changes in light transmission.

A

Automated Tray

41
Q

What is the principle used in BD Phoenix Automated Microbiology System?

A

Nephelometry

42
Q
  • Fully automated with AST
  • Uses disposable panels and is meant to be read every 20 minutes
  • Detects gram negative bacilli
  • You may be able to identify the organism within 2 to 12 hours (though there are many available in 4 hours)
  • Has 45 biochemical reactions (fluorogenic, fermentative, chromogenic, and carbon source substrates)
A

BD Phoenix Automated Microbiology System

43
Q

Identifies most clinically significant bacteria and yeast and performs both quantitative and qualitative AST

A

VITEK System

44
Q

What are the six reagent cards for the VITEK System?

A
  1. GN Card
  2. GP Card
  3. BCL Card
  4. YST Card
  5. NH Card
  6. ANC Card
45
Q

Identify the reagent card.

Gram negative fermenting and non-fermenting bacilli

A

GN Card

46
Q

Identify the reagent card.

Gram positive cocci and non-spore forming bacilli

A

GP Card

47
Q

Identify the reagent cards.

Gram positive spore-forming bacilli

A

BCL Card

48
Q

Identify the reagent card.

Yeast

A

YST Card

49
Q

Identify the reagent card.

Neisseria, Haemophilus and other fastidious gram negative bacteria

A

NH Card

50
Q

Identify the reagent card.

Anaerobic bacteria

A

ANC Card

51
Q
  • Has 64 wells each containing an individual test substrate
  • Incubated at 35 C and monitors each card optically collection
  • AST both gram positive and negative organisms
A

VITEK System

52
Q

What does the VITEK System check or identify?

A
  • Changes in the absorbance
  • Changes in the turbidity
  • Color changes as pH changes
53
Q

T or F: The test reactions for VITEK is read every 30 minutes.

A

F

15 minutes

54
Q

T or F: Increased turbidity indicates susceptibility.

A

F

Resistance, not susceptibility

55
Q

MICs is based on what two factors?

A
  1. Growth curve
  2. Slope of the curve
56
Q

What principles does MicroScan Walk Away (Siemens) utilize?

A

Colorimetric and Fluorometric

57
Q

T or F: The MicroScan Walk Away System is a semiautomated computer-controlled system.

A

F

Fully automated (kaya nga walk away e)

58
Q
  • Has a 40 or 96 panel capacity model
  • Can identify organisms in either two hours or 4 hours
  • Can take 4 to 7 hours for AST
A

MicroScan Walk Away (Siemens)

59
Q

What is the timeframe used in MicroScan Walk Away when the results given are converted to 15 digit codes?

A

2-Hour Identification (+ Fluoroscent Label)

60
Q

What do we call the 4-hour identification of MicroScan Walk Away (Siemens)?

A

Rapid Colorimetric System

61
Q

How many hours does it take the MicroScan Walk Away to perform AST?

A

4 to 7 hours

62
Q

This measures the degree of fluorescence.

A

Fluorometer

63
Q

What tool does the AST for MicroScan Walk Away (Siemens) use?

A

Colorimetric-Spectrophotometer

64
Q

Less fluorescence = ?

A

Susceptibility

65
Q

More fluorescence = ?

A

Resistant

66
Q

If you see this card

A

Wala edi may mata ka, congrats hehe.

Clinical Bacteriology (22 decks)

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