osmosis terminology Flashcards

1
Q

what is a negative control?

A

when doing an experiment the testing condition leads to an outcome is no different from what you would expect normally
e.g. Ecoli+glucose+amoxycillin=ecoli death

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2
Q

what is a positive control?

A

when doing an experiment the testing condition leads to an outcome that has a predicable amount of effect
e.g. ecoli+glucose+ new AB= variable cell death

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3
Q

what is an independent variable?

A

is the cause of change in the experiment e.g. the AB in the ecoli experiment

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4
Q

what is the dependent variable?

A

is the effect of the experiment e.g. the glucose in the experiment

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5
Q

what is a solute?

A

it’s a substance dissolved into another substance, usually the lesser amount e.g. sugar water

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6
Q

what is osmosis?

A

the passive transport of water from one solute to another between a selectively permeable membrane until equilibrium is reached

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7
Q

what is osmolarity?

A

the total concentration of all dissolved substances
Osmolarity (Osm l-1)=concentration (M) x number of ions solute dissociates into

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8
Q

what is concentration of solutes measured in?

A

Molarity (M)

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9
Q

what is ICF?

A

intracellular fluid (fluid stored inside the cytosol)

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10
Q

what is ECF?

A

extracellular fluid (stored outside of the cell)

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11
Q

what does isotonic mean?

A

when the ICF and ECF is balanced to the net amount of water

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12
Q

what is hypotonic?

A

when the ECF is lower in concentration than the ICF or
When the ICF is higher in concentration than the ECF
i.e. more water entering the cell than leaving it

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13
Q

what is hypertonic?

A

when the ICF is lower than the EF or
when the ECF is higher than the ICF
i.e. more water is leaving the cell than entering it (crenation)

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14
Q

what is crenation?

A

when the cell starts to shrink due to dehydration

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15
Q

what is lysis?

A

when the cell is swollen and the membrane bursts due to too much water

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16
Q

what is the ratio of blood to solution for the Haematocrit experiment?

A

1:3 sheep:solution

17
Q

what is the biological mechanism that causes dehydration?

A

Osmosis: the ECF becomes hypertonic so the ICF starts to crenate to regain equilibrium.

18
Q

what is the formula to calculate the volume of solute in solution?

A

C1V1=C2V2