Oncology Flashcards
Cytology indication
Lesion palpable external/ seen on image
Organomegaly
Cavitary effusion
Cancer staging
Pyrexia of unknown origin
Cyto expectations
Inflammation/ neoplasia
Benign/ malignant
Can’t do-
Hyperplasia/ adenoma
Cyto adv/ dis
Advantages -
non invasive
inexpensive equipment
done in house
rapid results
Dis -
May not be definitively diagnostic
Need sample to be of diagnostic quality
Slide evaluations
Macroscopic- label and smeared correctly
Micro
-10x scan whole slide, find most representative area
- higher magnification to ID cells and morphology
Sample quality problems
Thickness
Insufficient staining time
Inadequate drying
-Nuclei don’t pop- more staining needed
-bubbles- aspiration pressure, repeat with needle only technique
-cells balled up- slow drying
Inflammation or neoplasia
Inflammation
-inflammatory cells, microbes, fibroblasts
Neoplasia
-monomorphic atypical cells
-abnormal cells for site of collection
Neoplasms can become inflamed
-SCC, mammary, mast cell
Inflammation types
Neutrophilic
-imm-mediate, pathogens
Histio/macrophage
- FB, fungus, Mycobacterium
Eosinophilic
-hypersensitivity, parasites, cancer
Lympo/lymphoplasmacytic
- antigenic stimulation, Chr inflammation
Benign tumour cyto
Uniform
Delineated
Low mitotic count
Low nuclear: cytoplasmic ratio
Malignant neoplasia cyto
Pleomorphism
Anisokaryosis: Atypical nuclear: cytoplasmic ration
High and varied mitotic count
Clumped chromatin
Multinucleation
Epithelial neoplasms
Tight clumped cells
Distinct cytoplasmic borders
Sebaceous adenoma
Raised cauliflower alopecic lesions
Foamy cytoplasm
Perianal gland tumour
Entire males
Sheets of hepatocyte like cells
Large oval cells
Squamous cell carcinoma
Proliferative and ulcerative lesions
Limbs, pinnae, faces
Polygonal cells, pleomorphic
Highly malignant and invasive
Mammary carcinoma
Background blood, basophilic
Variable cellular arrangement
Pleomorphic
Mesenchymal tumours
Poor delineation
Soft tissue sarcoma
-fibro, lipo, leiomyo, rhabdmyo, perivascular
=
Lipoma
Benign
Can’t distinguish from normal subcut adipose tissue on cytology
Osteosarcoma
Round/ spindle cells
Basophilic cytoplasm
Eccentrically placed nucleus and prominent nucleoli
Osteoclasts - large multinucleated cells may be present
Round cell tumours
Cells exfoliate individually
Round cells with distinct borders
Ex
- transmissible veneral, mast cell, lymphoma, plasmacytoma
Histiocytoma
Dome shaped, alopecia
Younger dogs, regresses after few weeks
Small lymphocytes common
Plasmacytoma
Cutaneous plasmacytoma is benign
Deep blue cytoplasm
Round eccentric nuclei
Lymphoma
Round
High nuclear: cytoplasm ration
Large lymphocytes
Monomorphic population
Mast cell tumour
Breed related - boxer/ pug
Vary in granularity
less granular -> more aggressive
Eosinophils often seen aswell
(Neuro) endocrine tumour
Exfoliate into loose sheets
Naked nuclei in lakes of cytoplasm
Highly cellular samples
Ex-
-thyroid, phaeochromocytoma
Thyroid carcinoma
Blood in background
Clusters cells with scattered naked nuclei
Extracellular pink colloid often seen