mt.1 Antigen Antibody Lec 6 (IgG) Flashcards
Where are isotypic differences located?
within the constant regions of the heavy chain
*IgG, A, M, E, D
Where are allotypic differences located?
within some portion of Fc region
- **doesn’t affect binding to antigen
- IgG from one person may be different than IgG from another person within Fc
Where are idiotypic differences located?
Differences are with the Fab region
- **variable regions
- IgG from one immune response to antigen A is different than IgG from another immune response to antigen B and etc
Five Immunoglobulin classes of antibody based on heavy chain constant region
Y heavy chain= IgG class alpha haeavy chain= IgA class U heavy chain = IgM class epsilon HC= IgE sigma HC= IgD class
What are the two classes of lights chains?
Kappa
Lambda
T/F… any individual immunoglobulin molecule will have kappa or lambda
TRUE. They will have one OR the other. THEY WILL NOT HAVE BOTH
T/F… any population of immunoglobulins will have both kappa and lambda represented
TRUE. every population will have both light chains represented, but individual immunoglobulins will have one or the other
T/F… Each immunoglobulin isotype is identical
FALSE
*Each immunoglobulin isotype has distinctive features
What immunoglobulin has the highest concentration in blood?
IgG
IgG heavy chains have how many domains?
4 (one variable, 3 constant)
IgG heavy chains is how big?
150,000 daltons mw
What are the sub classes of IgG?
four sub-classes: IgG1, IgG2, IgG3, IgG4
Most sub-classes of IgG are able to fix ____, cross the _____, and enhance ______?
fix complement
cross placenta
enhance phagocytosis (opsonization)
complement fixation requires the _____ domains?
Fc domains
IgG antibodies are good at opsonization, this means?
enhancement of phagocytosis
The Fc domains of IgG bind to?
Fc receptors
Fc receptors are found on?
neutrophils and macrophages
usually phagocytic cells interact with pathogens via? Antigens allow macrophages to do what?
toll-like receptors (NOT specific)
* antibody allows macrophage to find pathogens that have already caused response (memory0
Fc receptors are typically called?
Fc GAMMA receptors
order of highest concentration of Igs in blood?
G > A > M > D > E
Does IgG have a long half life?
YES. The longest, which is good because it’ll take awhile to wash out of your system
two characteristics shared by immuglobulin super family?
1) Ig domain is 110AA long
2) intrachain di-sulfide bonds to give 2D loop characteristics
* evolutionarily thought to start as a single domain but with different variable domains bc other proteins have similarities
