Monoclonal Antibody

Question 1

Monoclonal antibodies are developed by ______ and ______

Answer 1

Kohler and Milstein

Question 2

Purified antibodies cloned from single cells in order to bind to a specific antigen

Answer 2

Monoclonal antibody

Question 3

Hybrid cells

Answer 3

Hybridoma

Question 4

Cells that reproduce indefinitely and synthesize antibodies

Answer 4

Hybridoma

Question 5

Hybridoma cells is due to the fusion of ___________ and ________ cells

Answer 5

Hybridoma: Activated B cells + Cancer cell (myeloma cell)

Question 6

Old medium used to induce fusion of activated b cells + myeloma cells

Answer 6

Sendai Virus

Question 7

New: Induces fusion of plasma cells with myeloma cells, producing a hybridoma

Answer 7

Polyethylene glycol (PEG)

Question 8

Three populations after fusion using PEG

Answer 8

Hybridoma cells
Spleen Cells
Myeloma Cells

Question 9

A selective medium that will allow the selective growth of Hybridoma cells ONLY

Answer 9

Hypoxanthine, aminopterin, and thymidine (HAT) medium

Question 10

Why does hybridoma cells, survive in a HAT medium?

Answer 10

Due to transferase (HGRPT) and thymidine kinase pathway from the spleen cells which have the ability to synthesize nucleotides

Question 11

Steps in Monoclonal Antibody

1. Removal of spleen from the mouse
2. Place mixture of fused cells in HAT
3. Immunization with antigen to the mouse
4. Fusion of b cells with myeloma cells in PEG
5. Removal of RBCs from spleen and Isolating B cells
6. Identify hybridoma cells that produces antibody of interest

Answer 11

3 > 1 > 5 > 4 > 2 > 6

Question 12

the presence of __________ blocks the other pathway which cells makes DNA from new nucleotides

Answer 12

Aminopterin