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Immunology-Serology > Introduction to Serology > Flashcards

Introduction to Serology Flashcards

1
Q

Serological tests are based on _________________

A

Antigen-antibody complex reaction

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2
Q

Blood specimens should be separated form the serum promptly within ________ minutes from the clot after centrifugation

A

30-60 minutes

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3
Q

Routine Clotting is in what _____ temperature

A

Room temperature

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4
Q

Delayed Processing

Routine:
Within 72 hrs:

A

Delayed Processing

Routine Refrigerate (4-6C)
Within 72 hrs: Freeze serum (-20C)
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5
Q

Inactivation of Serum is done in to ______________

A

Inactivation of complement/destroy complement activity

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6
Q

Ways of Complement Inactivation

Routine Inactivation:
Rapid Inactivation:
Others:

A

Ways of Complement Inactivation

Routine Inactivation: 56C for 10 minutes
Rapid Inactivation: 61C for 3 minutes
Others: Aging, Agitation, Chemical Treatment

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7
Q

Reasons for complement Inactivation

A

C1q may cause false + syphilis results
C1 may cause false + Passive/Latex agglutination
MAC may cause false + hemolysis reaction

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8
Q

HBV are killed within ______

A

10 minutes

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9
Q

HIV are killed within _______

A

2 minutes

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10
Q

_____ survive 7 days in dry surface

A

HBV

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11
Q

HIV survive ____ days in dry surface

A

3 days

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12
Q

HIV survive ____ days in wet surface

A

7 days

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13
Q

Two types of Collection in serology

A

Acute Phase

Convalescent Phase

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14
Q

Acute phase is when the disease is first discovered or suspected within ______ after onset

A

4 days; 5-15 days

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15
Q

Convalescent phase is within _____ after onset

A

2 weeks

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16
Q

Central concept of serologic testing

A

Antibody Titer

17
Q

________ is the reciprocal of the highest dilution which antibody is still detectable

A

Antibody Titer

18
Q

Types of Serologic Reaction

A

Primary Reactions
Secondary Reactions
Tertiary Reaction

19
Q

Primary Reactions

A

EIA
RIA
FIA

20
Q

Primary reactions is a ________ reaction of soluble complex that needs _____ to indicate a reaction

A

Primary reactions is a NON-VISIBLE reaction of soluble complex that needs LABEL to indicate a reaction

21
Q

Secondary Reactions

A 
Agglutination
Precipitation
Floculation
Neutralization
CFT
Toxin-antitoxin assay
22
Q

Secondary Reaction a _______ reaction the do not need labels to indicate reaction due to presence of insoluble A-g0Ab complex

A

Secondary Reaction a VISIBLE reaction the do not need labels to indicate reaction due to presence of insoluble A-g0Ab complex

Immunology-Serology (15 decks)

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