Introduction to Serology

Question 1

Serological tests are based on _________________

Answer 1

Antigen-antibody complex reaction

Question 2

Blood specimens should be separated form the serum promptly within ________ minutes from the clot after centrifugation

Answer 2

30-60 minutes

Question 3

Routine Clotting is in what _____ temperature

Answer 3

Room temperature

Question 4

Delayed Processing

Routine:
Within 72 hrs:

Answer 4

Delayed Processing

Routine Refrigerate (4-6C)
Within 72 hrs: Freeze serum (-20C)

Question 5

Inactivation of Serum is done in to ______________

Answer 5

Inactivation of complement/destroy complement activity

Question 6

Ways of Complement Inactivation

Routine Inactivation:
Rapid Inactivation:
Others:

Answer 6

Ways of Complement Inactivation

Routine Inactivation: 56C for 10 minutes
Rapid Inactivation: 61C for 3 minutes
Others: Aging, Agitation, Chemical Treatment

Question 7

Reasons for complement Inactivation

Answer 7

C1q may cause false + syphilis results
C1 may cause false + Passive/Latex agglutination
MAC may cause false + hemolysis reaction

Question 8

HBV are killed within ______

Answer 8

10 minutes

Question 9

HIV are killed within _______

Answer 9

2 minutes

Question 10

_____ survive 7 days in dry surface

Answer 10

HBV

Question 11

HIV survive ____ days in dry surface

Answer 11

3 days

Question 12

HIV survive ____ days in wet surface

Answer 12

7 days

Question 13

Two types of Collection in serology

Answer 13

Acute Phase

Convalescent Phase

Question 14

Acute phase is when the disease is first discovered or suspected within ______ after onset

Answer 14

4 days; 5-15 days

Question 15

Convalescent phase is within _____ after onset

Answer 15

2 weeks

Question 16

Central concept of serologic testing

Answer 16

Antibody Titer

Question 17

________ is the reciprocal of the highest dilution which antibody is still detectable

Answer 17

Antibody Titer

Question 18

Types of Serologic Reaction

Answer 18

Primary Reactions
Secondary Reactions
Tertiary Reaction

Question 19

Primary Reactions

Answer 19

EIA
RIA
FIA

Question 20

Primary reactions is a ________ reaction of soluble complex that needs _____ to indicate a reaction

Answer 20

Primary reactions is a NON-VISIBLE reaction of soluble complex that needs LABEL to indicate a reaction

Question 21

Secondary Reactions

Answer 21

Agglutination
Precipitation
Floculation
Neutralization
CFT
Toxin-antitoxin assay

Question 22

Secondary Reaction a _______ reaction the do not need labels to indicate reaction due to presence of insoluble A-g0Ab complex

Answer 22

Secondary Reaction a VISIBLE reaction the do not need labels to indicate reaction due to presence of insoluble A-g0Ab complex