Introduction to Serology

Question 1

How can you consider that a test is a Serological Test?

Answer 1

It involves ANTIGEN-ANTIBODY REACTION

Question 2

Primary Reaction vs. Secondary Reaction

Description: ______________
Tests: _________________

Answer 2

Primary Reaction

Description: DIRECTLY measure Ag-Ab interaction; Non-visible Reaction - Label
Tests:
EIA
RIA
Chemiluminescence 

Secondary Reaction 
Description: Consequence of Ag-Ab reaction - Visible Reaction - No Label
Tests:
Agglutination
Precipitation
Neutralization
Complement fixation Test
Flocculation

Question 3

Specimen Preparation (Serum)

Tube: \_\_\_\_\_\_\_\_\_\_\_
Clotting: \_\_\_\_\_\_\_\_\_
Separate: \_\_\_\_\_\_\_\_\_\_
Centrifuge: \_\_\_\_\_\_\_\_\_
Storage (Delayed): \_\_\_\_\_\_\_\_

Answer 3

Specimen Preparation (Serum)

Tube: Red Top/Gel Separator
Clotting: 1 hr @ room temperature
Separate: 30 mins - 1 hr
Centrifuge: 10 mins
Storage (Delayed): Ref - 4C - 6C

Question 4

Inactivation of Complement - destroys complement activity

30 mins: _______
3 - 4 mins: ________

Answer 4

30 mins: Heat @ 56C

3 - 4 mins: 60C - 62

Question 5

Reasons for Complement Inactivation

1. C1q causes False ________ for Syphilis testing
2. C1 causes False (+) for ______________
3. C56789 causes False (+) _____________

Answer 5

1. C1q causes False (+) for Syphilis testing
2. C1 causes False (+) for Passive/Latex Agglutination
3. C56789 causes False (+) hemolysis reactions

Question 6

Monoclonal Ab vs. Polyclonal Ab

Specificity: ____________
Reactivity: _____________
Animal used: __________

Answer 6

Monoclonal Ab

Specificity: Specific
Reactivity: Non Cross-reactive
Animal used: MICE

Polyclonal Ab

Specificity: Non-specific
Reactivity: Cross reactive
Animal used: RABBIT