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HSCI 300 > Module 12: Immunological Testing Methods Obj. 5 > Flashcards

Module 12: Immunological Testing Methods Obj. 5 Flashcards

1
Q

4 main groups of labels used in immunoassays

A

Radioactive
Enzyme
Fluorescent
Chemiluminescent

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2
Q

Radioimmunoassay (RIA)

A

utilize atoms with unstable nuclei that emit radiation that can be detected using a counter

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3
Q

Enzyme immunoassay (EIA)

A

utilize non-isotopic labels

Enzyme labeled Ab or Ag may be used

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4
Q

Fluorescent Immunoassay (FIA)

A

molecules which will absorb the energy of a photon of one wavelength and emit a photon of a slightly longer (lower energy) wavelength

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5
Q

Chemiluminescence immunoassay (CIA)

A

substrates are modules which emit a photon of light as a result of a chemical reaction
ex. Luminol

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HSCI 300 (30 decks)

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