Microscopy Flashcards

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1
Q

Define an object

A

The real object or sample that you’re looking at

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2
Q

What is an image

A

It is what you look at when you look in the microscope

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3
Q

What is magnification

A

How many times larger the image is then the object

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4
Q

What is the equation for magnification

A

Magnification= image size/object size

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5
Q

Resolution

A

A measure of how detailed the image is

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6
Q

How does a microscope work?

A

Light from the room hits the mirror and reflects upwards through the object then it passes through the objective lens to the eye piece then to the eye

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7
Q

What is the positives and negatives about the light microscope

A

Positives
They are easy to use
They are relatively cheap
Small

Negatives
They require light
They have a resolution of 0.2 micrometers (not good to study the sub-cellular structures)

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8
Q

What are the positives and negatives of electron microscopes

A

Positives
You can see sub-cellular structures
The resolution is 0.1 nanometres
(2000 X better than light microscope)

Negatives
They are quite big
They are expensive

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9
Q

Order these smallest to largest
Micrometer,Nanometre,Kilometre, Millimetre, Metre

A

Nanometre
Micrometer (μ)
Millimetre
Metre
Kilometre

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10
Q

How big are atoms

A

0.1-0.5 nanometres

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11
Q

Size of viruses and bacteria

A

They are in micrometers

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12
Q

What’s the smallest thing a human can see

A

Width of a human hair

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13
Q

One centimetres to millimetre

A

10mm

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