MICROBIOLOGY - Stains

Question 1

What stain is used to identify fungi? (5)

Answer 1

1. Gomori-methamine silver - stains black
2. Grocott hexamine silver - fungi + acanthomoeba stains black
3. Periodic acid schiff - stains purple
4. Calcufluor - stains white
5. haematoxylin and eosin stains pink

Question 2

What stain is used to identify calcium (2)

Answer 2

Alizarin Red
Von Kossa

Question 3

What are the stains involved in dystrophies?
1) Reis-Bucklers
2) Granular Dystrophy
3) Macular dystrophy of cornea

Answer 3

1) Masson Trichome stains new collagen blue
2) Masson Trichome stains hyaline material red
3) Alician blue stains mucopolysaccharides

Question 4

What do MacConkey agar and McCoy agar grow?

Answer 4

1. MacConkey agar - lactose fermenting G-ves
2. McCoy agar - obligate intracellular bacteria (chlamydia

Question 5

What does Thayer-Martin medium and Thioglycate agar grow?

Answer 5

1. Thayter martin - G-ve cocci (Neisseria)
2. Thioglycate: aerobes on surface, anaerobes inside

Question 6

What does Lowenstein-Jenson medium grow?

Answer 6

Mycobacteria tuberculosis

Question 7

What does chocolate agar, blood agar and cooked meat both medium grow?

Answer 7

1. Chocolate - haemophilus, neisseria, moraxella
2. Blood agar - all bacteria except H, N, M above
3. Cooked meat broth - anaerobes and fastiduous organisms

Question 8

What does non-nutrient agar with E.coli grow and Sabouraud agar grow?

Answer 8

1. Non-nutrient - Acanthomaeba
2. Sabouraud - Fungi