IMMUNOLOGY - Basic (B-cells/T-cells, Immunoglobulins, MHC) Flashcards
Which immunoglobulin passes through the placenta to assist foetal immunity?
IgG
What is the main immunoglobulin in human tears?
IgA
What receptors do macrophages have? (3)
- pattern recognition receptors which recognise microbial components
- surface receptors that recognise Fc portion of immunoglobulin and complement components. Binding of these receptors to their ligands enhances phagocytosis of antigen (opsonisation)
- MHC class II molecules on surface when activated - present antigen to helper T-cells. Secrete IL-12 which directs T-cell differentiation to Th1 subset.
What pro-inflammatory cytokines do macrophages produce?
IL-1, IL-6, and TNF
Which complements are involved in the terminal pathway (membrane attack complex) and its deficiency predisposes to Neisserial infections?
C5-C9 (C3 is also associated)
IL-2 is produced by what cells?
CD4+ cell
HLA Class I Antigens vs
HLA Class II Antigens
Class I : all nucleated cells, allows CD8+ cytotoxic T cells to recognise and eliminate virus infected cells
Class II: macrophages, dendritic cells, B cells, endothelial cells, allows initiation of immune response by interaction with T-helper CD4+ cells
difference in structure between class 1 and class II HLA
picture
What cytokines do TH1 produce?
What cytokines do TH2 produce?
- TH1 : IFN-gamma, IL2 –> B cells, NK cell and macrophage activation
- TH2: IL3, IL4, IL5, IL6 –> mast cell and eosinophil activation
What are the main functions of interferon? (3)
- activation of NK cells and macrophages
- directive antiproliferative action on tumour and virus infected cells
- increased expression of Class 1 HLA on infected/tumour cells
What cells produce interferon?
T lymphocytes & macrophages
Which immunoglobulin is the first antibody to appear in infection?
IgM
What pathway does IgA activate?
Activates the alternative complement pathway (unlike other antibodies that activate the classical pathway)
What are the main types of eicosanoids? (3)
- Prostaglandins
- Leukotrienes
- Thromboxanes
What is the role of thromboxanes? (3)
vs
What is the role of leukotrienes? (3)
thromboxanes:
vasoconstriction, platelet aggregation and bronchoconstriction
leukotrienes
Bronchoconstriction, vasocontriction and increased vascular permeability
What is the cyclo=oxygenase pathway?
See diagram.
What is the effect of prostaglandin I2?
causes vasodilation and reduced platelet adhesion
What are the effects of prostaglandin E2? (5)
- vasodilation
- bronchodilation
- Release of pituitary hormones, adrenal cortex steroids and insulin from pancreas
- Macrophage activation
- Uterine contraction
What are antigens? Definition
molecules (sugars, proteins or glycoproteins) that are immunogenic (cause immune cell proliferation) and reactive (cause production of antibodies)
What are the main secretion chemicals from mast cells? (3)
What is their main function?
histamines, leukotrienes and prostaglandins
- They work on plasma endothelial cells to cause p-selectin protein translocation to the cell surface –> monocyte/neutrophil margination/activation into interstitial space.
- endothelial cell contraction which creates spaces between endothelial cells which allows plasma leakage through the pores into interstitial place to cause swelling/oedema (INCREASED VASCULAR PERMEABILITY)
- Nociceptor activation to cause pain (usually by bradykinins)
- smooth muscle relaxation –> vasodilation (localised hyperaemia)
What is the function of phospholipase A2?
breaks down phospholipids into arachidonic acid
What do macrophages secrete?
- IL-1 and TNF-alpha –> activates plasma endothelial cells cause selectins to translocate to endothelial cell surface to cause monocyte and neutrophil activation.
- IL-8 binds to receptor on endothelial surface cell membrane –> ICAM/VCAM proteins on cell surface membrane –> monocyte/neutrophil activation