GENETICS - Basics Flashcards

1
Q

What is the process of DNA replication (4)

A
  1. DNA helix (parent strand) unwound by DNA helicase to form two single strands (templates)
    at the replication fork
  2. DNA polymerase adds nucelotides to these templates to allow new DNA leading strand to grow in 5’ to 3’ direction
  3. DNA lagging strand formed by discontinuous short nucleotide strands called Okazaki fragments by a different DNA polymerase which is formed in the 3’ to 5’ direction
  4. Thees are joined together by DNA ligase
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2
Q

What is transcription? What enzyme is involved?

A

DNA code is read in nucleus and complementary messenger RNA is produced, which leaves for the cytoplasm for protein synthesis.

RNA polymerase in a 5 prime to 3 prime direction

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3
Q

What is the structure of mitochondrial DNA? What type of inheritance? Which genes does it code for?

A
  1. Circular double stranded-DNA
  2. Inherited from mother
  3. Encodes 37 genes that produce structural RNA (22 tRNA, 2rRNA) & Proteins of electron transport chain (complex I, III, IV and V)
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4
Q

What is the relation between histones and DNA?

A

histones are alkaline positively charged proteins, allow them to interact with negatively charged DNA

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5
Q

Which is the short arm chromosome and which is the long arm chromosome?

A

p - petit
q - long

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6
Q

What is the structure of a nucleotide (3)

A
  1. Deoxyribose (pentose sugar)
  2. Nitrogen base (purines and pyramidines)
  3. Phosphate Group
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7
Q

How are adjacent nucleotides linked to each other?

How are the DNA strands linked to each other?

A
  1. 3’ to 5’ phosphodiesterase bond
  2. Hydrogen bonds between bases.
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8
Q

DNA complexes are called?

A

Histones

Histones packed together to make chromatin.

Chromatin fibre pack together to form a chromosome.

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9
Q

Whats the difference between euchromatin and heterochromatin? (2)

A
  1. Euchromatin is less densely packed than heterochromatin
  2. Euchromatin contains acetyl group
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10
Q

How long does a cell cycle last?

A

Usually 24 hours

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11
Q

What is the G0 stage?

A

Cell is quiescient, some cells permanently stopped in G0

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11
Q

What is the M stage?

A

Mitosis - occupies one hour

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12
Q

What happens in G1 stage?

A

Biosynthetic activity, cellular contents are duplicated (excluding chromosomes)

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13
Q

What happens in S phase?

A

DNA synthesis and chromosomal replication to form two daughter chromatids (6-8 hours)

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14
Q

What happens in G2 stage?

A

Second gap phase with preparation for division (cell doubles mass, chromosome checks for errors)

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15
Q

What are the four stages of mitosis?

A

Prophase
Anaphase
Metaphase
Telophase (Cytokinesis)

16
Q

What happens in prophase? (4)

A
  1. Nucleolus disappears
  2. Chromatin condenses into chromosomes
  3. Separation of centrosomes
  4. Formation of mitotic spindle
17
Q

What happens in metaphase?

A

chromosomes align in the equatorial plane

18
Q

What happens in anaphase? (3)

A
  1. Centromere divides with chromatid separation at the centromere
  2. Chromatids move to the opposite poles of the cell

3.Unattached microtubules push against each other to elongate cells

19
Q

What happens in telophase? (4)

A
  1. New nuclear envelope forms
  2. Chromosomes unfold back into chromatin
  3. Nucleolus reappears
  4. Cells continue to elongate
20
Q

What happens in cytokinesis?

A

Division of cytoplasm

21
Q

What are the role of cyclins in the cell cycle?

A

Regulatory subunits within cells which regulate cyclin-dependent kinases (CDKs)

they drive the cell through stages of the cell cycle via phosphorylation of protein substrates

22
Q

Whats the difference between mitosis and meiosis?

A

see picture

23
Q

Which is the most error prone phase in meiosis?

A

Anaphase I

24
Q

What are the steps involved in protein transcription?

A

Initiation
1. Binding of transcription factors to promoter sequence in DNA (TATA box)

  1. RNA polymerase binds to promoter and separates DNA strands

Elongation
1. RNA polymerase along the DNA template strand adds complementary RNA nucleotide to the 3’ end of the RNA strand.

  1. The RNA molecule is made from the 5’ end to the 3’ end

Termination
3. RNA polymerase keeps transcribing until meets termination signal,

25
Q

What three processes are involved in pre-mRNA processing?

Where does it occur?

A
  1. Addition of 5’ cap to the beginning of RNA (7-methylguanine nucleotide addition)
  2. Addition of 3’ poly A nucleotide tail to end of RNA (aids transport and provides stability)
  3. RNA splicing - removal of introns at the spliceosome and pasting together of exons

This occurs in nucleus - leaves once becomes mature and goes towards ribosomes for translation.

26
Q

What is the process of translation?

A

Initiation
1. Start codon (AUG - methionine) is recognised
2. mRNA at 5’ end binds to small 40s subunit of ribosome, then large 60s subunit binds to make initiation complex.

Elongation
1. tRNA with initiator is attached to P site of ribosomal unit - tRNA/mRNA linkage is catalysed by initiation factor 2

  1. new tRNA with anticodon attaches to complementary codon of mRNA (amino acid is on other end of anticodon).
  2. Hydrogen bonds form between the two amino acids
  3. Once bond has formed, empty tRNA exits P site and ribosomal unit translocates one unit across to next codon and process repeats.
  4. Polypeptide chain is built from N terminal to C terminal

Termination
1. Stop codon enters A-site - no tRNA instead it is a release factor binds to A site

  1. This blocks further tRNA from binding and releases polypeptide chain into cytoplasm
27
Q

What are histone proteins?

A

Histones are proteins that have DNA wound around it, they are influenced by environmental change and dictate which DNA parts are open for transcription –> CONTRIBUTES TO EPIGENETICS.

28
Q

What is the Hardy Weinburg Equilibrium?

A

describes the constancy of gene frequences from one generaton to the next

29
Q

What is the criteria for Hardy Weinburg principle to apply? (4)

A
  1. Absence of mutation
  2. Absence of genetic drift
  3. Absence of natural selection
  4. Randomly breeding population
30
Q

Which bases are purines?

A

Adenine, Guanine, Hypoxanthine

31
Q

Which bases are pyrimidines?

A

Uracil, cytosine, thymine