MAJOR CLINICAL ENZYMES IIi

Question 1

more specific in acute pancreatitis

a. amylase
b. lipase
c. ggt

Answer 1

lipase

• more specific in AP
• not affected by renal disorders

Question 2

which of the ff methods uses a triolein as a subtrate

a. wacker et al
b. oliver rosalki
c. cherry crandal
d. saccharogenic method

Answer 2

Lipase
methods of determination

I. Cherry crandal method
- substrate : OLIVE OIL
modifications : used TRIOLEIN as a substrate

II . turbidimetric
more raid and simpler

III. colorimetric method

Question 3

which of the ff enzymes acts on a substrate in only in emulsified form

a. amylase
b. starch
c. lipase
d. acp

Answer 3

lipase

• enzymes that hydrolyzes glycerol esters of long chain fatty acids
• acts on a substrate in only in emulsified form
• Full activity in the presence of bile salts amd colipase

Question 4

newborn screening marker

a. G6PD
b. Cholinesterase
c. GGT
d. ACE

Answer 4

G6PD

Question 5

important role in diagnosis and management of organophosphate poisoning

a. G6PD
b. Cholinesterase
c. GGT
d. ACE

Answer 5

Cholinesterase

• important role in diagnosis and management of organophosphate poisoning ( insecticise / pesticide posioning) low CNS
• used to monitor the effect of muscle relaxant after surgery

Question 6

which of the following uses only Acetylcholine as a substrate

a. TRUE cholinesterase
b. PSEUDO cholinesterase

Answer 6

TRUE cholinesterase
USES ONLY acetylcholine

PSEUDO
hydrolyzes a VARIETY of choline esters

Question 7

which of the following found in serum and in the white matter of the central and peripheral nervous tissue

a. TRUE cholinesterase
b. PSEUDO cholinesterase

Answer 7

true choline

• primary location are synapses of nerve cells.
• also seen in RBCs, lung, brain and spleen

Pseudo choli

• found in serum and in the white matter of the central and peripheral nervous tissue
• also in heart , liver and pancreas