analyticsl methods Flashcards
measurement of light transmitted by a solution
a. spectrophotometer
b. nephelometer
c. reflectometry
d. flame photometry
spectrophotometer
-measurement of light transmitted by a solution
what theory of light waves is photons travelling in waves
a. electromagnetic radiation
b. wavelength
c. amplitude
d. visible light
theory of wavelength
- wavelength
- PHOTONS travelling in waves
- wavelength
- distance between TWO PEAKS
- amplitude
- distance between PEAK AND TROUG
what theory of light waves is distance between PEAK AND TROUG
a. electromagnetic radiation
b. wavelength
c. amplitude
d. visible light
theory of wavelength
- wavelength
- PHOTONS travelling in waves
- wavelength
- distance between TWO PEAKS
- amplitude
- distance between PEAK AND TROUG
beer lambert’s law
states that the concentration of a substance is (directly / inversely ) proportional to the amount of light
beer lambert’s law
states that the concentration of a substance is DIRECTLY proportional to the amount of light or INVERSELY proportional to the logarithm of the transmitted light
what is the most common source of light for work in the VISIBLE OR NEAR INFRARED REGIONS
a. tungsten light bulb
b. deuterium lamp
c. xenon lamp
d, mercury arc lamp
light source
> 2 types
- Incandescent tungsten or tungsten iodide lamp
- most common SOURCE of light for work in the VISIBLE OR NEAR INFRARED REGIONS - Deuterium - discharge lamp and Mercury arc lamp
- most commonly USED for UV work
WHAT IS THE MOST COMMONLY USED MONOCHROMATORS
a. colored glass filters
b. prism
c. diffraction gratings
d. interference filters
diffraction gratings
- most commonly used monochromators
which of the compponent of spectrophotometer is minimize unwanted/ stray light
a. light source
b. entrance slit
c. exit slit
d. pmt
entrance slit
- minimize unwanted/ stray light
- prevent the entrance of scattered light into monochromator system
what kind of monochromator is least expensive, simple, and not precise but useful
a. filters
b. prism
c. diffraction gratings
filters
- least expensive
- simple
- not precise but useful
what is the commonly used cuvet
a. aluminum silica
b. quartz
c. borosilicate
aluminum silica
which of the compponent of spectrophotometer detects and converts transmitted light into photoelectric light
a. light source
b. entrance slit
c. exit slit
d. pmt
photodetector
- detects and converts transmitted light into photoelectric light
- detects the amount of light that passes through the sample in th ecuvet
measure the quantity of light reflected by a liquid sample
a. spectrophotometer
b. reflectometry
c. aas
d. flame photometry
reflectometry
- measure the quantity of light reflected by a liquid sample that has been dispensed onto a grainy or fibroud solid support
application
> urine dipstick analysis
> dry slide chemical analysis
measurement of concentration is done by detecting the absorption of electromagnetic by atoms rather than molecules
a. spectrophotometer
b. reflectometry
c. aas
d. flame photometry
atomic absorptionspec
- detecting absorption of electromagnetic by ATOMS RATHER THAN MOLECULES
application:
>measurement of UNEXCITED TRACE METALS (CALCIUM AND MAGNESIUM)
meaurement of light emitted atoms
a. spectrophotometer
b. reflectometry
c. aas
d. flame photometry
flame photometry
- measure of light emitted by EXCITED ATOMS
Application:
> widely used to determine the concentration of SODIUM , POTASSIUM AND LITHIUM
WHAT IS THE MOST COMMON LIGHT SOURCE IN FLUOROMETRY
A.TUNGSTEN LIGHT BULB
B. DEUTERIUM LAMP
C. XENON LAMP
fluorometry
- contain fluorescing molecules
components
> XENON LAMP - most common light source
application
> meausre SMALL PARTICLES SUCH A SDRUGS
which of the following has application of detection of bacterial growth and bacterial culture, coagulation studies
a. flame photometry
b. , aas
c. fluorometry
d. turbidimetry
turbidimetry
- light blocked
applications:
> detection of bacterial growth and bacterial culture
> antibiotic sensitivity
> coagulation studies
> protein concentration in urine and csf
