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clinical chemistry > LIVER FUNCTION TESTS > Flashcards

LIVER FUNCTION TESTS Flashcards

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1
Q

how many blood does liver receives for minutes

a. 25mL
b. 180mL
c. 15mL
d. 10mL

A
  • the liver is the CHIEF METABOLIC ORGAN OF THE BODY
  • It receives 15ml OF BLOOD PER MINUTE
  • it is composed of 2 types of cells
    HEPATOCYTES
    KUPFER CELLS (phagocytic)
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2
Q

important in assessing nutritional status and presence of severe diseases involving liver, kidney and bone marrow

a. total protein
b. globulin
c. bilirubin
d. albumin

A

TOTAL PROTEIN

  • NOT required FASTING
  • important in assessing nutritional status and presence of severe diseases involving liver, kidney and bone marrow
  • TP and ALBUMIN is 10% higher in ambulatory individual
  • plasma levels of tp is 0.2 to 0.4g/dl higher than serum due to fibrinogen
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3
Q

which of the ff statement is correct

a. plasma levels of tp is 0.2 to 0.4g/dl higher than serum due to fibrinogen
b. serum levels of tp is 0.2 to 0.4g/dl higher than plasma due to fibrinogen
c. plasma levels of tp is 0.2 to 0.4g/dl lower than serum due to fibrinogen
d. serum levels of tp is 0.2 to 0.4g/dl lower than plasma due to fibrinogen

A

TOTAL PROTEIN

  • NOT required FASTING
  • important in assessing nutritional status and presence of severe diseases involving liver, kidney and bone marrow
  • TP and ALBUMIN is 10% higher in ambulatory individual
  • PLASMA levels of tp is 0.2 to 0.4g/dl HIGHER THAN SERUM due to fibrinogen
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4
Q

transudates have TP of __________

a, <3g/dl

b. >3g/dl
c. >0.2 g/dl
d. <0.2 g/dl

A

TRANSUDATES have TP of <3.0 g/dL (<50% of the serum TP)

EXUDATES have >3g/dl

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5
Q

which of the ff methods in TP is the standard reference method but not routinely used

a. Kjeldahl method
b. Biuret method
c. Folin Ciocalteu method
d. Ultraviolet Absorption method

A

Kjeldahl method

  • standard reference method but not routinely used
  • based on measurement of the nitrogen content protein
  • uses SERUM treated with TUNGSTIC ACID forming PROTEIN FREE FILTRATE (PFF)
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6
Q

1 GRAM of nitrogen is equivalent to how many grams of PROTEINS

a. 16.8 grams
b. 1. 64 grams
c. 2. 64 grams
d. 6.54 grams

A

Kjeldahl method

  • standard reference method but not routinely used
  • based on measurement of the nitrogen content protein
  • uses SERUM treated with TUNGSTIC ACID forming PROTEIN FREE FILTRATE (PFF)
  • 1g of nitrogen = 6.54g of proteins
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7
Q

what is the end product in Kjeldahl method

a. H2SO4
b. Sulfuric acid
c. ammonia
d. ethanol

A

kjeldahl method

reagent : H2SO4 (sulfuric acid)

end product : Ammonia (NH3)

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8
Q

which of the ff methods in TP is the most widely used method

a. Kjeldahl method
b. Biuret method
c. Folin Ciocalteu method
d. Ultraviolet Absorption method

A

Biuret method

  • most widely used method, recommended by IFCC expert panel
  • requires 2 peptide bonds and alkaline medium
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9
Q

which of the following reagent does not belong to Biuret method

a. NH3
b. alkaline copper sulfate
c. rochelle salt
d. NaOH and potassium iodide

A

Biuret method

reagent : NARs
NaOH and potassium iodide
alkaline copper sulfate
Rochelle salt

Kjeldahl method
reagent : H2SO4
endproduct : NH3

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10
Q

which of the ff methods in TP has the highest analytical sensitivity

a. Kjeldahl method
b. Biuret method
c. Folin Ciocalteu method
d. Ultraviolet Absorption method

A 
Folin ciocalteu (lowry) method
  - highest analytical sensitivity

Kjeldahl method
- reference method

Biuret method
- most widely used

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11
Q

which of the ff TP has a principle of OXIDATION OF PHENOLIC COMPOUNDS CAN GIVE A DEEP BLUE COLOR

a. Kjeldahl method
b. Biuret method
c. Folin Ciocalteu method
d. Ultraviolet Absorption method

A

Folic ciocalteu method

principle : OXIDATION OF PHENOLIC COMPOUNDS such as tyrosine , tryptophan and histidine CAN GIVE A DEEP BLUE COLOR

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12
Q

which of the ff TP has a principle CUPRIC IONS COMPLEX THE GROUPS INVOLVED IN THE PEPTODE BOND FORMING VIOLET COLORED CHELATE

a. Kjeldahl method
b. Biuret method
c. Folin Ciocalteu method
d. Ultraviolet Absorption method

A

BIURET METHOD

PRINCIPLE: CUPRIC IONS COMPLEX THE GROUPS INVOLVED IN THE PEPTODE BOND FORMING VIOLET COLORED CHELATE

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13
Q

In Ultraviolet Adsorption method, adsorption at 280nm is due to Tryptophan , tyrosine and __________

a. histidine
b. pehnylalanine
c. nota

A

Ultraviolet Adsorption method

  • protein absorb light at 280 and 210 nm
  • adsorption at 280nm is due to Tryptophan , tyrosine and PHENYLALANINE
  • adsorption at 210nm is due to absorbance of peptide bonds at that wavelength
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clinical chemistry (26 decks)

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