M3. Factors affecting enzyme activity

Question 1

Measures the rate at which an enzyme converts substrate to products

Answer 1

Enzyme Activity

Question 2

4 factors that affect the Enzyme activity:

Answer 2

1. pH
2. temperature
3. substrate concentration
4. enzyme concentration

Question 3

• charge on acidic & basic amino acids at the active site.
• it denatures & causes loss of catalytic functions of enzymes
• causes protonation & deprotonation of substrate

1. pH at which an enzyme exhibits maximum activity
2. physiological pH ranges from
3. maintain the optimum pH for an enzyme

Answer 3

pH

1. optimum pH
2. 7.0-7.5 [7.4]
3. biochemical buffers

Question 4

1. active in the stomach, functions best at pH ____.
2. it has a pH of 4.6
3. active in the small intestines, functions best at pH ____.
4. it has a temperature of 65C

Answer 4

1. pepsin; 2
2. salivary amylase/ptyalin
3. trypsin; 8
4. papain

Question 5

• Measure of kinetic energy
• the higher the temperature.
• the faster the movement.
• the frequent the collisions.
• unreversible activity of an enzyme

Temperature at which an enzyme exhibits maximum activity

Answer 5

Temperature

optimum temperature

Question 6

1. Increased of ____ will obtain the enzyme activity
2. measures the number of substrate molecules transformed per minute; in the conditions of?

Answer 6

1. substrate concentration
2. turnover number
   - pH
   - temperature
   - saturation

Question 7

1. substrate concentration increases = enzyme concentration increases
2. substrate and enzyme concentrations are?

Answer 7

1. saturation kinetics
2. directly proportional

Question 8

• kept in low number because they are not consume.
• as it becomes greater, the reaction rate also increases. [directly proportional w/ reaction rate].

Answer 8

enzyme concentration