M2.6. Translation. Flashcards
a process in which mRNA codons are deciphered to synthesize a protein molecule
Translation
- an rRNA–protein complex
- serves as the site of protein synthesis
Ribosome
Ribosomes contain:
- 4 rRNA molecules
- 80 proteins packed into two rRNA-protein subunits (one small and one large)
Ribosomes are?
1. ____ % rRNA
2. ____ % protein by mass
- 65%
- 35%
A ribosome’s active site -
I don’t get this part :<
Large subunit
a RNA catalyst
Ribosome
binds to the small subunit of the ribosome.
mRNA
Five Steps of Translation Process?
AIE TP
- Activation of tRNA
- Initiation of protein synthesis
- Elongation
- Termination
- Post-translational processing
addition of specific amino acids to the 3’-OH group of tRNA.
Activation of tRNA
Begins with binding of mRNA to small ribosomal subunit such that its first codon (initiating codon AUG) occupies a site called the P site (peptidyl site)
Initiation of protein synthesis
Adjacent to the P site in an mRNA–ribosome complex is A site (aminoacyl site) and the next tRNA with the appropriate anticodon binds to it.
Elongation
The polypeptide continues to grow via translocation until all necessary amino acids are in place and bonded to each other.
Termination
gives the protein the final form it needs to be fully functional
Post-translational processing
the first codon [initiating codon AUG] occupies a site called?
what amino acid is present in AUG?
P site/ Peptidyl site
methionine
P site is adjacent to?
A site/ Aminoacyl site