Lecture 8

Question 1

Primary purpose of fermentation

Answer 1

Regenerate NAD for reuse.

The electron acceptor is an organic molecule.

Question 2

Secondary purpose of fermentatoion

Answer 2

Generate additional energy
Energy yields are very small

Question 3

Acids taste ____.

Answer 3

sour

Question 4

All fermentation pathways will use an ______ compound as an electron acceptor.

Answer 4

organic

Question 5

What is the biologically speaking best electron acceptor?

Answer 5

Oxygen

Question 6

What are the common features in many ETS pathways?

Answer 6

• NADH Oxidase
• Quinones
• Cytochromes

Question 7

Quinones are _______ lipids and carry both ______ and ______.

Answer 7

Isoprenoid lipids, protons, electrons

Question 8

Iron sulfer proteins contain ___-____ iron, are acid sensitive, and are _____ carriers only.

Answer 8

non-heme, electron carriers

Question 9

Cytochromes have _____ groups and are _____ carriers only.

Answer 9

heme, electron

Question 10

Flavoproteins are common proteins with _____ cofactor, either ____ or ____. They carry two ___ and two ____.

Answer 10

flavin, FAD or FMN, two protons, two electrons

Question 11

End result of Mitochondiral respiratory ETS

Answer 11

driving protons out of cytoplasm

Question 12

Proton Motive Force (PMF) equation

Answer 12

PMF = (delta)p = (delta) (psi) - 60(delta)pH

Question 13

What does delta psi represent?

Answer 13

Change in electrical gradient

Question 14

PMF can be used to

Answer 14

• create ATP
• drive flagellar rotation
• push ions into and out of cell

Question 15

Symport

Answer 15

Same direction as proton movement

Question 16

Antiport

Answer 16

Opposite direction as proton movement

Question 17

____ totation drives the ____ subunit shaft.

Answer 17

F0 (F not), F1

Question 18

F1F0ATP synthase synthesizes ____ from ____ + ____

Answer 18

ATP from ADP + Pi