13

Question 1

Meischer

Answer 1

Nuclein

Question 2

Griffith

Answer 2

Transformation

Question 3

Avery, MacLeod, McCarty

Answer 3

Transformation

Question 4

Hershey and Chase

Answer 4

Blender experiment

Question 5

Chargaff

Answer 5

There are as mnay As as Ts and as many Cs as Gs

Question 6

Base
Sugar

Answer 6

Nucleosides

Question 7

Base
Sugar
Phosphatee

Answer 7

Nucleotides

Question 8

What direction do nucleotides go?

Answer 8

5’ to 3’

Question 9

How are the nucleotides numbered?

Answer 9

Base: numbers
Sugar: number ‘ (prime)

Question 10

What is attached to 1’?

Answer 10

The base

Question 11

What is attatched to the 5’?

Answer 11

Deoxyphosphate

Question 12

What is attatched to the 3’?

Answer 12

A hydroxyl group

Question 13

What is significant about 2’?

Answer 13

2’ is either oxygenated or deoxygenated. If there is no oxygen, it is deoxyribose and is for DNA. If there is an oxygen, then it is ribose and is for RNA

Question 14

Are purines or pyrimidines bigger?

Answer 14

purines are larger

Question 15

A and G

Answer 15

purines

Question 16

C, U, and T

Answer 16

pyrimidines

Question 17

How any hydrogen bonds to AT and GC make?

Answer 17

AT = 2
CG = 3

Question 18

Where does DNA replication begin?

Answer 18

oriC

Question 19

What enzyme performs most of the DNA synthesis during replication?

Answer 19

DNA plymerase III

Question 20

After two round of replication in light nitrogen, Meselson and Stahl observed what forms of DNA?

Answer 20

Intermediate and light

Question 21

After one round of replication in light nitrogen, Meselson and Stahl observed what forms of DNA?

Answer 21

Intermediate

Question 22

After three rounds of replication in light nitrogen, Meselson and Stahl observed what forms of DNA?

Answer 22

Intermediate and light (more light than intermediate)

Question 23

Genome

Answer 23

the complete DNA sequence

Question 24

Genotype

Answer 24

specific DNA sequence

Question 25

Phenotype

Answer 25

Appearance/behavior

Question 26

Prokaryotes are ____ and ____ (mostly)

Answer 26

circulat and haploid

Question 27

positive supercoiles, ___ winding

Answer 27

over winding

Question 28

negative supercoils, ___ winding

Answer 28

under winding (easier to open)

Question 29

Loader places ___ at each end of origin

Answer 29

helicase

Question 30

___ ____ needs a free 3'OH

Answer 30

DNA polymerase

Question 31

___ begins replication

Answer 31

Primase

Question 32

____ strand follows helicase

Answer 32

leading

Question 33

Gaps filled in by DNA Plymerase ____

Answer 33

DNA polymerase I

Question 34

___ seals nicks

Answer 34

ligase

Question 35

DNA polymerase __ is involved with repair

Answer 35

DNA polymerase II

Question 36

____ are actually stationary

Answer 36

replisomes

Question 37

Extrachromosomal pieces of DNA

Answer 37

plasmids

Question 38

____ plasmids have one or two copies per cell

Answer 38

low-copy-number plasmids

Question 39

___ plasmids have up to 500 copies per cell.

Answer 39

high-copy-number plasmids

Question 40

Low-copy-number plasmid example

Answer 40

f plasmid of e. coli pbr22

Question 41

Plasid replication stating at nick bound by RepA protein

Answer 41

unidirectional

Question 42

Plasmid gene examples

Answer 42

- antibiotic resistance - encoding poteins to metabolize rare food sources - Virulence genes to allow pathogenesis - allow symbiosis