Lecture 2 Flashcards
Gastrulation
cell movement from ball into 3 layered structure known as gastrula
invagination
sheet of cells moving inward by apical constriction of actin fibers
ingression
individual cells leave an epithelial state/sheet and become freely moving (mesenchymal)
mesenchymal
freely moving cells
epiboly
sheet of cells thinning out to become longer
intercalation
rows of cells moving in between each other to become longer
ingression is similar to
this process is similar to that used by carcinoma cells to extravasate and metastasize
three layers of cells
ectoderm, mesoderm and endoderm
cadherins are
cell adhesion molecules
some things in ECM (extracellular matrix) are
collagen, proteoglycans, glycoproteins
Xenopus leavis
frog
What ,in frogs, triggers the opening of a pore that allows cells on outside of embryo to move inside?
Spemann’s organizer
what is the hole formed by Spemann’s organizer?
blastopore- in frogs only
Where does blastpore form?
dorsal lip of blastocyst
Transcription factor expressed in the dorsal marginal region that is involved in starting gastrulation in frogs
goosecoid
explain basic steps of activation of gastrulation
receptors attach to cells and get activated by ligand
starts signal transduction cascade in cytoplasm
leads to activation of transcription factors in the nulceus and gene expression
examples of glycoproteins in extracellular matrix
fibronectin, chondronectin, tenascin
outer ectoderm cells move by what to move into cell in frogs
epiboly (sheet movement by thinning out)
first thing to form from mesoderm
notochord from chorda mesoderm
types of mesoderm
notochord,
PARAXIAL MESODERM (somites) INTERMEDIATE MESODERM (kidneys and gonads) LATERAL MESODERM (splanchnic (internal organs, blood endothelium) and somatic (limb skeleton, ventral skin dermis and connective tissue of body cavity)
A blastula of a chick is in what shape
blastodisc
Inner cell membrane turns into
epiblast and hypoblast
groove in bird and mammal gastrulation similar to blastopore in frog
primitive streak
This induces the formation of the primitive streak in bird
koller’s sickle
structure in birds that is similar to Spemann’s organizer, that triggers cells to enter center of blastocyst
hensen’s node
cells in birds and mammals move into cell in a ___ way
mesenchymal
Mammals do not need large yolk sac because of this ___
placenta
placenta is made from these cells
trophoblast
structure in mammals, that is similar to Spemann’s organizer, that triggers cells to enter center of blastocyst
node
once gastrulation completes primitive steak regresses and leaves ___ behind
notochord
notochord is made of what type of cells
mesoderm
process by which ectoderm forms a neural plate and folds into neural tube
neurulation
embryonic precursor to the central nervous system (brain and spinal cord)
neural plate which turns into neural tube
explain neurulation
notochord stimulates ectoderm to thicken into the neural crest and invaginate onto itself creating the neural tube.
neural crest cells change states and travel all over body to form bones, melanocytes and neurons
what causes neurulation
BMP
what inhibits neurulation
Noggin/ chordin
what is the name of the process through which the notochord causes neurulation
neural induction
neural tube closure in chicks happens at **
level of future midbrain and zips up in either direction
neural tube closure in mammals happens
at multiple locations
the last regions of the neural tube to close are
cranial and caudal neuropores
lethal condition when anterior/ cranial neuropore does not close correctly
anencephaly
condition when caudal neuropore does not close correctly
spina bifida cystica
neural tube changes into
forebrain, midbrain and hindbrain
another name for forbrain
prosencephalon
another name for midbrain
mesencephalon
another name fir hindbrain
rhombencephalon
neural tube defect where skin fails to separate from the spine in rhodesian ridgebacks
dermoid sinus
duplication of what factors make rhodesian ridgebacks more predisposed to dermoid sinus
FGF3, FGF4, FGF19 and ORAOV1
rhodesian ridgeback breeders are not allowed to breed ___ with ___
ridged with ridged
two examples of cell adhesion molecules
cadherins and CAMS
example of cell junction molecule
gap-junctions-connexins
what is EMT , give example
epithelial to mesenchymal
neural crest cells