Lecture 1: Introduction to Veterinary Mycology Flashcards
What is mycotoxicosis
Intoxication with fungal byproducts that may contaminate feed
Alfatoxins and ergot alkaloid are examples of ___
Mycotoxins
What are the 3 phyla of fungi with veterinary importance
- Ascomycota
- Basidomycota
- Zygomycota
What predisposes an animal to fungal invasion
- Immunosuppression
- Prolonged antibiotic therapy
- Traumatized tissue
- Persistent moisture/poor ventilation
T or F: fungi are eukaryotes
True
What are the 2 basic morphological forms of fungi
- Yeast- round to oval cell often with budding cells
- Mold- filamentous, multicellular body organisms
Identify which is yeast vs mold
left: yeast
Right: mold
What are 2 important cell membrane components of fungi
- Ergosterol
- Chitin
What is the predominant sterol in cell membrane of fungi
Ergosterol
What drugs target the Ergosterol of fungi
Amphotericin B and azoles
What is chitin
Polysaccharide providing cell wall rigidity
What specific fungal structure does the yeast cryptococcus have
Encapsulated
What specific fungal structure does the yeast Candida albicans
Germ tubes as well as pseudohyphae
What are dimorphic fungi and what is an example
Temperature dependent switch between yeast (37 C) and mold (25 C)
Ex: histoplasma
Identify the fungal yeast structures 1-3 and provide examples of yeasts that have those structures
- Encapsulated (ex: cryptococcus)
- Germ tubes (ex: Candida albicans)
- Pseudohyphae (ex: Candida albicans)
Based on the morphology, what type of yeast is this
cryptococcus
Based on the morphology, what type of yeast is this
candida
What are hyphae
Long filament or strand of cells +/- septa
What is a mycelium
Mat of hyphae
Is aspergillus septate or non-septate
Septate
Is rhizopus and mucor septate or non-septate
Non-septate
What are sporangiospores
An asexual spore type produced within a sporangium
What is a sporangiphore
Stalk upon which the sporangium sits
Label the fungal structures 1-3
- Sporangia/um
- Sporangiophore
- sporiangiospores
What are conidia
An asexual spore type that is open and not enclosed in a sporangium
What is a conidiophore
Bears the vesicle, metula and Philade with conidia
What type of spore type is this
conidia
What type of spore type is this
sporangiospore
Identify 1-5
- Vesicle
- Conidiophore
- Conidia
- Phialide
- Hyphae
What is this
aspergillus
What are macroconida
Large conidia with identifiable structures
What are microconidia
Small conidia, less specific
micro or macro conidia
Micro
Micro or macro conidia
macro
What this
microsporum
What this
Trichophyton
What are blastoconidia/ blastospores
Budding yeast
What type of conidia is this
Blastoconidia/ blastospores
What type of conidia is this
arthroconidia
What type of conidia does coccidiodes immitis have
Arthroconidia
What are the 2 ways for spores to undergo asexual reproduction
- Sporangiospores- within sporangium
- Conidia- from conidiophore
What classifies a fungal disease as Mycoses
Granulomas +/- necrosis +/- abscess formation
What classifies a fungal disease as mycotoxicoses
Intoxication and tissue damage, not an active infection and not a mycosis
What classifies a fungal disease as a hypersensitivity
Allergic pneumonitis, etc. may occur but are not classified as mycoses or mycotoxicoses
What classifies as fungal disease as superficial. what are some examples
Typically involved skin, hair, nails (ex: microsporum and other dermatophytes)
What classifies a fungal disease as subcutaneous. what are some examples
Deep dermal, often resulting from trauma
(Ex: sporothrix and mycetomas)
What classifies a fungal disease as opportunistic. what are some examples
Seen in immunocompromised patients (ex: mucocutaneous candidiasis, cryptococcosis)
What classifies a fungal disease as systemic/endemic. what are some examples
Severe, often regionally distributed (ex: coccidiodes, blastomyces, histoplasma)
What is an endothrix infection
Fungal invasionto the surface of the hair shaft, intact cuticle
What is an ectothrix infection
Fungal attachment of hair shaft, cuticle degrading
Which fluoresces under Wood’s lamp: endothrix or ectothrix
Ectothrix
Endothrix and ectothrix infections are classified as what type of fungal disease
Superficial mycoses
Most fungi are obligate ___
Aerobes
What are the temperature requirements for fungi to grow
Ambient (25 C), +/- body temperature
Most fungi can handle high ___conditions and pH < __
Osmotic, <5
What is a common fungal media that inhibits bacterial growth
Sabaroud dextrose
Which grows faster: yeast or mold
Yeast
What is required to ID yeast
Biochemical +/- molecular ID (MALDI, PCR)
What is required to ID mold
Based on structure +/- molecular ID
What are some ways to exam yeast or mold under microscope
- Tape preparation
- Stained tissue
What is used for tape preparation
Lactophenol cotton blue
What tissue stains can be used on mold and yeasts
PAS, GMS