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Fungal/Virology > LEC 6 - Opportunistic Fungal Infections > Flashcards

LEC 6 - Opportunistic Fungal Infections Flashcards

1
Q

What is an important step in the life cycle of Phyium insidiosum?

A

Needs to infect plants to complete cycle

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2
Q

What allows P. Insidiosum to invade animal tissue?

A

Motile zoospores w/ germ tube production

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3
Q

Affected Animals: P. Insidiosum

A 
Horses
Dogs 
Calves 
Sheep 
Cats
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4
Q

Lesions: P. Insidiosum

A

Granulomatous tissues - Subcutaneous or Intestinal

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5
Q

Infective stage: P. Insidiosum

A

Animal wades in water

Zoospore infects = oppurtunistic

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6
Q

How doe the zoospores of P. Insidiosum invade the tissue?

A

Through the germ tubes that produce aseptate hyphae

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7
Q

What does P. Insidiosum do while its disseminating?

A

Causes thrombosis

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8
Q

What causes tissue damage with P. Insidiosum infections?

A

Degranulating EOS + Mast cells

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9
Q

Where do lesions in horses tend to occur with P. Insidiosum infections?

A

Body parts that contacted the water

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10
Q

Disease: P. Insidiosum

A

Cutaneous pythiosis

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11
Q

Lession appearance: P. Insidiosum in Horse

A

Large, circular granulomatous nodules
Ulcerate
Pruritus
Necrotic yellowish coral-like mass

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12
Q

What can happen with chronic P. Insidiosum infections?

A

Bone involvement

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13
Q

Define: Enteric pythioss

A

Stenotic fibrous GI lesions

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14
Q

Target Organs: P. Insidiosum in DOgs

A

Stomach + SI

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15
Q

Clinical Signs: P. Insidiosum in Dogs

A

Vomiting
Wt. Loss
Diarrhea
PALPABLE ABDOMINAL MASS

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16
Q

What is causing the abdominal mass in a dog with a P. Insidiosum infection?

A

Extension into pancreas + bile ducts + mesenteric LN

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17
Q

Where do cutaneous lesions in dogs tend to occur with P. Insidiosum infections?

A

Base of tail
Extremities
Perineum
Ventral Neck

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18
Q

Diagnosis: P. Insidiosum

A 
Tissue stain 
Immunofluorescence 
PCR 
Culture 
Serology
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19
Q

Culture: P. Insidiosum

A

Sabouraud dex. agar

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20
Q

ID colony: P. Insidiosum

A

Aseptate hyphae

Colorless to white w/ short aerial hyphae

21
Q

Stain: P. Insidiosum

A

PAS + Methenamine silver

22
Q

P. Insidiosum: Treatment Dogs

A

Aggressive surgery
Remove infected bowl
Amputate distal limbs infected
NO ERGOSTEROL IN MEMBRANE - still txt w/ Itraconazole

23
Q

P. Insidiosum: Treament Horses

A

Wide surgical excision + Immunotherapy

24
Q

Describe: P. Insidiosum Immunotherapy

A

Regulation of T cells from Th1 to Th2

25
Q

Describe P. Insidiosum Thermotherapy

A

Apply local heat daily to lesion

Enhances intracellular killing capability of NEU

26
Q

Morphology: Sporothrix schenickii

A

Dimorphic temperature dependent fungus

27
Q

Etiology: S. Schenickii

A

Worldwide

28
Q

Culture for Environmental form :S. Schenickii

A

SDA

29
Q

Colony: S. Schenickii

A

Hyphae + Condiophores w/ rosette clusters of pear shapped conidia

30
Q

Environmental form: S. Schenickii

A

25 deg

Hyphae + Condiphores

31
Q

Infective form: S. Schenickii

A

37 deg

Cigar-shapped pleomorphic budding yeast cells

32
Q

Culture for Infective form: S. Schenickii

A

Brain Heart Infusion Agar `

33
Q

Clinical Signs: S. Schenickii = Horse/Dogs

A

Cutaneous/Lymphocytaneous form

34
Q

Clincal Signs: S. Schenickii = Cats

A

Lymphocutaneous

Disseminated

35
Q

Describe: Equine Sporotrichosis

A

Lymphocutaneous
Spores enter skin abrasion
Nodules develope along superficial lymphatic vessesl

36
Q

Describe Feline Sporotrichosis

A

Primary nodular lesions
Ulcerative with seropurulent exudate
Extends to lymphatics

37
Q

Areas of infection: S. Schenickii - Felines

A

Limb extermities
Head
Tail

38
Q

Area most infected with S. Schenickii in cats

A

Brazial

39
Q

Describe Canine Sporotrichosis

A

Multiple cutaneous lesions
Ulcerated + Crusted + Alopecic
Occasional lymphocutaneous involvement

40
Q

Treatement: S. Schenickii Horse

A

Systemic Isodid preparation
Organic perferred (EDDI)
Feed additive

41
Q

Treatment: S. Schenickii - Dogs/Cats

A

Itraconazole

42
Q

Infection form: P. Carinii

A

Asexual trophozoite

43
Q

Reproducing form: P. Carinii

A

Sexual reproducing cyst

44
Q

Predisposition: P. Carinii

A

High prevalence of young animals

Airborne/transplacental

45
Q

Animals infected: P. Carinii

A 
Dogs 
Horses
Rabbits 
Cats 
Pigs
46
Q

DOC: P. Carinii

A

Trimethoprim
– or –
TMS
Supportive care

47
Q

Culture: P. Carinii

A

CAN NOT BE

48
Q

PCR: P. Carinii where from?

A

lung tissue

49
Q

Clincal signs: P. Carinii

A

Diffuse Pulmonary consolidation
Marked thickening alveolar space
Proteinaceous exudate in alveoli

Fungal/Virology (26 decks)

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