Labs Flashcards
Effect of heat on quarternary proteins
Disrupts hydrophobic interactions and dipole-dipole interactions
Thiol reducing agent on proteins
Disrupts disuphide bonds
SDS on proteins
Is an anionic detergent.
It solubilises, denatures + coats proteins w/ -ve charge
What property allows SDS to dissolve hypdrophobic molecules?
Lipophillic tail
Conjugate
Combine with another molecule or join together
Pattern of absorbance measure
the particular wavelengths of light absorbed by conjugated bonds in a molecule
Deuterium lamps in spectrophotometry
Emit UV light so are suitable for measuring the absorbance of DNA, RNA and proteins.
Absorbance of UV light in proteins
Peptide bonds and aromatic rings in amino acids absorb UV light
Scanning spectroscopy
Uses a range of wavelengths when measuring absorbance.
When using a micropipette
1) Have appropriate tip on when touching liquid
2) Hold pipette up straight with liquid in
3) Take liquid in slowly so it doesnt ‘jump’ in the mechanism
Wavelength absorbed by DNA
~260nm
WAvelength absorbed by protein
~280nm
Absorbance measure at 280nm generally has absorbance equivalent to :
1mg of protein per imL of solution
Measuring protein quantification
A280 of 1 ~ 1g/L = 1mg/mL of protein
What causes bands to be fainter in electrophoresis?
Sample more diluted
Cell volume equals
(Pi x 6) x width^(2) x length
What causes lipid accumulation to increase with cell biovolume in algae?
As the cells generate and accumulate oil bodies within the cytosol in response to nitrogen starvation this causes and increase in cell volume, hence an increase in cell biovolume positively correlates with increased lipid content.
Why is oil immersion used in microscopy?
to increase the resolution of the image of the small cell.
When making a blood film, which cell type is most abundant?
Erythrocytes
Microcytic means someones red cells are…
Low mean corpuscular (cell) volume (MCV)
When identifying ABO blood groups, an individual showing agglutination w/ anti-B antisera only will have the following blood group
B
1fl equals
10^(-15) L
1pg equals
10^(-12) g
Blood pressure equals
BP = CO x TPR