Lab2

Question 1

what is an IC50

Answer 1

The molar concentration of competing ligand which reduces the specific binding of a radioligand by 50% of the amount bound in the absence of competitor

Question 2

Which statistical test should you use to compare the results of several groups that are repeated measurements on the same individuals at different times

Answer 2

One way ANOVA

Question 3

How would you improve the lung mechanics practical

Answer 3

• Larger sample
• Allow recovery period after each test
• Allow longer to adjust to spirometer before recording

Question 4

When measuring FVC, FEV and PEFR, why is only the highest value for each result used?

Answer 4

1) ‘Practice effect’ - volunteer unfamiliar with equipment

2) Highest value takes into account possible nervousness in prior recordings.

Question 5

Why should limb be relaxed or supported when measuring pulse?

Answer 5

If muscles are contracted then muscle spindles twitch – this may be
mistaken for the pulse

Question 6

When looking for differences in sets of 2 matched pairs measurements, which ST?

Answer 6

Paired T-test or Wilcoxon matched pairs

Question 7

When looking for differences in sets of 2 unmatched pairs measurements, which ST?

Answer 7

Unpaired T-test or mann whitney U