Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

BIO 442 > Lab Practical 1 > Flashcards

Lab Practical 1 Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

Serratia marcescens gram pos or neg

A

negative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Endospore stain method

A

Cover slide with paper towel and saturate with malachite green
Steam for 5 minutes
Rinse
Safranin
Rinse
Blot

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

spirit blue test/lipase, which pos and neg?
s. aureus
e.coli

A

s. aureus positive
e. coli negative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What were the two organisms tested in the catalase test and what was the result for each?

A

s. aureus- catalase positive
l. lactis- negative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

From the carbohydrate metabolism lab which organism could be classified as a non fermenter?

A

pseudomonis aeroginosa

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What does the citrate test, test for?

A

Differentiate between members of Enterobacteriaceae.
The ability to use citrate as a carbon source and perform citrate fermentation. Contain citrate permease.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What bacterial cell structure does the Gram reaction test?

A

peptidoglycan layer in cell wall

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

gram stain method

A

Crystal Violet
rinse
Iodine
rinse
Alcohol
Rinse
Safranin
Rinse
Blot

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

decarboxylase test, which pos and neg?
p. merabilis
e.coli

A

e. coli postive
p. merabilis negative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Staphylococcus aureus gram pos or neg?

A

positive

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

geletin hydrolysis test, which pos and neg?
b.cereus
e.coli

A

b. cereus positive
e. coli negative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

m. phlei acid fast pos or neg?

A

postive

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

why did e. coli have a green sheen in the EMB?

A

ferments lactose with acid production

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

starch hydrolysis test, which pos and neg?
b.cereus
e.coli

A

b. cereus positive
e. coli negative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

For the decarboxylase test: describe the general reaction of this test, what does a positive result look like and why does the indicator dye bromocresol purple change color with a positive result? What was added to the top of the tube and why?

A

Testing for the ability to turn on the decarboxylase gene. A positive result is purple and changes due to the high pH due to a build up of amines. Mineral oil is added to block oxygen and induce fermentation.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What is EMB agar?

A

selective agar for gram negative coliforms

17
Q

acid fast stain method

A

carbolfuschin stain
rinse
acid-alcohol
rinse
methylene blue
rinse
blot

Heat or chemical mordant

18
Q

For MR test which bacteria had a positive result and what did it look like? What did it mean?

A

E. coli and bright red. Mixed acid producer.

19
Q

Which organisms are endospore formers?

A

bacillus subtilis and bacillus cereus

20
Q

s. aureus, acid fast pos or neg?

A

negative

21
Q

Explain how the gelatin test works and what is meant by a positive result.

A

Bateria with gelitanase introduced to gelatin will liquify gelatin.

22
Q

Bacillus cereus gram pos or neg?

A

positive

23
Q

For the indole production portion of the SIM test, which bacterias had a positive result and what did it look like?

A

K. oxytoca
E. coli
P. valgaris
pink layer on top when kovac’s reagent added

24
Q

What are the expected oxygen requirements for catalase-positive and catalase-negative species?

A

Positive: aerobic or facultative aerobe
Negative: anaerobe

25
Q

What does the acid fast stain work on?

A

mycolic acid in the waxy cell wall

26
Q

For the VP test, which bacteria had a positive result and what did it look like?

A

e. aerogenes, pinkish/red, fermentation with aceiton

27
Q

What are the arrangments for bacteria? 6

A

diplo- two cells
staphylo-cluster of cells
strepto-chain of cells
tetrad- four cells
sarcina- eight cells
plaisades- group of rods

28
Q

What is the positive and negative result for spirit blue? What is it testing?

A

Testing for the production of lipase
Positive: halo around growth
Negative: no halo

29
Q

For the sulfur production portion of the SIM test, which bacteria had a positive result and what did it look like?

A

P. valgaris, mostly black, H2S produced

30
Q

What does a change from red to yellow mean in the phenol red test? Why is it changing?

A

fermentation is occuring. Changes to yellow because acid is produced.

31
Q

Escherichia coli gram pos or neg?

A

negative

32
Q

What enzyme does an organism have if it is starch positive?

A

amylase

33
Q

Explain how the starch hydrolysis test is prepared and what do we add to read the test and why?

A

Use Starch agar and add iodine to allow us to view the halo around the colony.

34
Q

What is a positive and negative citrate test? At what pH does the color change happen?

A

Positive: blue
Negative: green
Turns blue at 7.6 (basic)

35
Q

Cell morphologies 7

A

coccus- ball
bacillus- rod
vibrio- curved rod
spirillum- cork screw
spirochete- wavy
coccobacillus- short rod
pleomorphic- multiple shapes

BIO 442 (26 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions