Lab Nitrogenous Bases

Question 1

Two classes of Nitrogenous Bases

Answer 1

Purines (A and G) and Pyrimidines (C, U, and T)

Question 2

The sugar is covalently linked to a nitrogen of the purine or pyrimidine

Answer 2

Nucleosides

Question 3

A hydroxyl group on the sugar of the nucleoside is covalently linked in an ester bond with phosphoric acid

Answer 3

Nucleotides

Question 4

Macromolecules in which the nucleotides are linked by phosphodiester bonds between 3’ and 5’ positions of the sugars

Answer 4

Nucleic Acids

Question 5

A=T and C=G

Answer 5

Ex) 10% A means 10% T and 40% G and 40% C

Question 6

Hydrolysis of DNA and RNA under controlled conditions yield

Answer 6

Nucleotides

Question 7

Further hydrolysis of nucleotides yield

Answer 7

Nucleosides

Question 8

Nucleosides degrade to

Answer 8

Sugar and Nitrogenous Bases

Question 9

Consists of two chains of polynucleotides

Answer 9

DNA

Question 10

DNA is stabilized by

Answer 10

H bonds between base pairs

Question 11

A blue compound is formed when DNA is treated with

Answer 11

Diphenylamine under acidic conditions

Question 12

Furfural in the presence of ferric chloride yields a green color when it reacts with

Answer 12

Orcinol

Question 13

RNA turns green when it reacts with

Answer 13

Orcinol

Question 14

DNA turns blue when it reacts with

Answer 14

Diphenylamine