Cell Bio Practical (Lab 1)

Question 1

Stains that help with viewing size, shape and arrangement

Answer 1

Basic Dye

Question 2

Basic stain

Answer 2

Positive Chromatophore

aka nuclear stains

Question 3

Acidic stain

Answer 3

Negative Chromatophore

aka Cytoplasmic stains

Question 4

Basic stain that dissociates

Answer 4

Methylene Blue Chloride

Question 5

Differential stain used to distinguish and differentiate

Answer 5

Gram Stain

Question 6

Gram Positive

Answer 6

Purple

Question 7

Gram Negative

Answer 7

Pink

Question 8

A basic dye, primary stain in Gram

Answer 8

Crystal Violet

Question 9

Acts as a mordant (a substance that increases the affinity of cells for the crystal violet dye)

Answer 9

Iodine

Question 10

Removes the dye from the cell

Answer 10

Alcohol (or acetone) called a Decolorizer

Question 11

A basic dye of a different color that is used to counter stain the cell if the crystal violet is removed by the decolorization solution

Answer 11

Safranin

Question 12

Ability to distinguish close together objects

Answer 12

Resolving Power

Question 13

the size of the cone of light the lens can receive

Answer 13

Numerical Aperature

Question 14

Attached to the rotating nosepiece, or turret, at the base of the body tube

Answer 14

Objectives lens

Question 15

The process of enlarging an object only in appearance

Answer 15

Magnification

Question 16

The ability to tell two points apart as separate points

Answer 16

Resolution

Question 17

The vertical distance with which structures are in sharp focus is called

Answer 17

The Depth of Focus

Question 18

The distance from the bottom of the microscope lens to the part of the specimen that is in focus

Answer 18

Working Distance