Cell bio Lab Final

Question 0

V=

Answer 0

Velocity

Question 1

V=Eq/f 
 What does each variable stand for? 
V=
E=
q=
f=

Answer 1

V=velocity
E=electric field
q=change of particle
f= frictional coefficient

Question 2

E=

Answer 2

Electric field

Question 3

q=

Answer 3

Charge of particle

Question 4

f=

Answer 4

Frictional coefficient

Question 5

V=Eq/f is the formula used for?

Answer 5

Gel electrophoresis

Question 6

A _______ is a closed circular, self replicating, extra chromosomal molecule of DNA found in some strains of bacteria.

Answer 6

Plasmid

Question 7

Define beer lambert law

Answer 7

At a specific wavelength of light the absorbance is proportional to the concentration of the solution and amount of light that is passed through.

Question 8

What three functions does the Ficoll loading dye serve in the agarose gel electrophoresis procedure?

Answer 8

• increases the density to allow even loading of sample
• adds color to allow visualization of sample as it moves and to simplify loading
• travels towards the anode at different predictable speeds

Question 9

It migrates through the agarose gel at approximately the same rate as a linear double stranded 300 base pair fragment of DNA

Answer 9

Bromophenol blue -300 base pairs

Question 10

Some bacteria display an ability to “take up” DNA at a particular stage in their life cycle. What method was used in the transformation laboratory to make bacteria capable of taking up DNA?

Answer 10

CaCl2 & heat shock

Question 11

Th rate at which the product is formed in the presence of acid phosphatase is compared to the rate at which product is formed in the absence of acid phosphatase is called

Answer 11

Enzymatic activity

Question 12

In the plasmid DNA transformation experiment the JM101 escherichia coli plasmid contained two genes. What protein did these genes encode?

Answer 12

Amp^r- Beta lactamase

Lac^z - Beta galactosidase

Question 13

When performing the agarose gel electrophoresis, ___________ was used as an intercalating agent, and _______ was used as a chelating agent.

Answer 13

Ethidium bromide

EDTA

Question 14

How many grams of agarose are needed to prepare a 2.9% gel when added to 325 ml of 1X TAE buffer?

Answer 14

2.9 X
—-= —– Proportion equation
100 325

9.43 grams

Question 15

_______ is a type of enzyme that responds to various metabolic conditions by changing their catalytic activity.

Answer 15

Regulatory enzymes

Question 16

8 millimoles/liter (mmol/L) = ________micromoles/Liters (micmol/L)

Answer 16

8.0*10^3

Question 17

What does the T, A, & E stand for in TAE buffer?

Answer 17

T- trismethylamine base
A-glacial acetic acid
E- EDTA (chelator)

Question 18

What would be the resulting 1:XX dilution using the following volumes?
37 microliters of solute and 444 microliters of solvent

Answer 18

37 37
___ = _____
37+444 481

=> 1:13

Question 19

A=ECL identify each variable

Answer 19

A=absorbance (unit less)
E= molar extinction coefficient (M^-1cm^-1)
C=concentration (M)
L=length (cm)

Question 20

The formula A=ECL is used for?

Answer 20

Spectrophotometry

Question 21

What is lambda phase

Answer 21

Edit wrong

Question 22

In the isolation of plasmid DNA, when 100microliters of neutralizing solution was added to the tube a white precipitant was formed. What did the white precipitant contain?

Answer 22

DNA and…?