Lab 3.9: Endospore stain Flashcards
Some bacterial species can differentiate into dormant cells called ??
-endospores
-happens when environmental conditions, such as nutrient depletion or high temps are unsuitable for growth.
Endospores are highly resistant to ?? and ??, which allows them to survive in this state for long periods of time
-heat and chemicals
The endospore stain is a ?? stain used to detect the presence, shape, and location of endospores in bacterial cells
-differential
What is present in the spore coat that resists staining, in which extreme measures must be taken to stain an endospore:
-keratin
Most common bacterial species that produce endospores (2);
-Bacillus
-Clostridium
Endospores may be located in 3 different areas of the cell:
-central (in the middle of the cell)
-subterminal (between end and middle of cell)
-terminal (at the end of the cell)
The size of the endospore might be: (2)
-spherical
-elliptical (oval shaped)
What is the primary stain?
-malachite green
What are cells decolorized with?
-water
What is the counterstain?
-safranin
Upon completion, endospores are ??, vegetative and spore mother cells are ??
-green
-red
Procedure steps (9);
-prepare heat fix smear
-add strip of bibulous paper to cover smear
-add malachite green and heat steam for 5-7 minutes
-rinse with water
-add safranin for a minute
-rinse with water
-blot dry (gently)
-observe under microscope (10X, 40X)
-add oil for 100X
Differential stains function to:
-Highlight differences between cells or parts of a cell.
Which of the following is a characteristic of endospores?
-They have a highly impermeable outer layer
They have a highly impermeable outer layer to facilitate…
the penetration of the primary stain into the endospore
Upon competition of the endospore staining procedure, the bacteria will appear:
-pink
Endospores are produced by bacteria to surivive:
-poor environmental conditions
True or False:
An endospore is a dormant form of the bacterium that allows it to survive poor/stressful environmental conditions.
-false
the Schaeffer-Fulton method is another name to which staining technique
-endospore stain
What is the primary stain used in endospore staining technique?
-malachite green
Which of the following is the decolorizing agent used in endospore staining technique?
-water
Which of the following is the counterstain used in endospore staining technique?
-safranin
Upon competition of the endospore staining procedure, the endospore will appear
-green
True or false:
Spores are resistant to heat and chemicals because of a tough outer covering made of the protein keratin.
-true
What is the purpose of heat in the endospore staining technique?
-to facilitate the penetration of the primary stain into the endospore
