Lab 2.7: Anaerobic jar Flashcards
Various airtight jars and pouches have been developed that allow cultivation of what kind of microorganisms?
-obligately anaerobic
-microaerophilic
-capnophilic microorganisms
Airtight jars and pouches vary in size, but they can carry ?? or more agar plates or test tubes and still fit in a standard incubator
-one
Each system can be used with a variety of ?? generating sachets (packets) that produce the desired ??
-gas
-atmosphere
The small white strip at the left is the indicator, white=
-no O2
Large white envelope is the ??
-gas generator packet
The GasPak 100 Anaerobic Jar is large enough to hold ?? petri dishes
-12
Methylene blue, which is ?? when oxidized (O2), turns ?? when the oxygen is removed
-blue
-colorless
Which of the following is a common method for cultivating anaerobic microorganisms in the laboratory?
-employing anaerobic jars
Anaerobic means that:
-they can not survive in the presence of oxygen
Which of the following is a common method for creating anaerobic conditions in the laboratory?
-Employing an anaerobic chamber or glove box
What is the role of anaerobic indicators in anaerobic chambers?
-to measure oxygen concentration
What type of metabolism do anaerobic microorganisms primarily rely on?
-fermentation
Which organisms do you grow in an anaerobic jar?
-anaerobic
What is the purpose of using reducing agents in anaerobic growth media?
-To create a reducing environment by removing oxygen
What kind of media do you to cultivate anaerobic bacteria:
-reducing
