Lab 1.4: Common Aseptic Transfers and Inoculation Methods

Question 1

Microbiologists must be able to transfer living microbes from one place to another aseptically meaning..

Answer 1

-without contamination of the culture, the sterile medium, and the surrounding’s

Question 2

To prevent contamination of the sample, inoculating instruments must be sterilized ….

Answer 2

-prior to use

Question 3

Culture:

Answer 3

-a medium that contains living microbes

Question 4

Pure culture:

Answer 4

-when a culture contains a single species

Question 5

How to label media:

Answer 5

-with the name, date, medium, and inoculum
-do it around the edge of plate

Question 6

Never hold a tube culture by its…

Answer 6

-cap

Question 7

How should you hold a inoculating loop or needle?

Answer 7

-like a pencil in your dominant hand

Question 8

What are the types of media?

Answer 8

-broth
-agar slants
-agar deep
-plated media

Question 9

The instruments used for transfers are: (2)

Answer 9

-inoculating loops
-inoculating needles

Question 10

Hold the text tube at an ?/ to minimize the opportunity for airborne contamination

Answer 10

-angle

Question 11

The broth culture is:
the agar slant culture is:
the agar plate culture is:

Answer 11

-serratia marcesence
-E.Coli
-Staphlococcus aureus

Question 12

Inoculating needle or loop?
broth=
plate=
slant=
deep=

Answer 12

-loop
-loop
-needle
-needle

Question 13

Aseptic technique means that that you perform the preparation of media or the transfer of living microbes:

Answer 13

-without introducing contamination

Question 14

True or False:
Plates are always labeled on their lids

Answer 14

-FLASE

Question 15

Look back on quiz to review the questions with pictures