5.14: Gelatin hydrolysis

Question 1

Gelatin is a protein derived from ??

Answer 1

-collagen (component of vertebrae tissue)

Question 2

Gelatinases are extracellular enzymes produce and secreted to hydrolyze

Answer 2

-gelatin
- can absorb amino acids and use them for metabloic purposes

Question 3

Bacterial hydrolysis of gelatin occurs in ?? sequential reactions:

Answer 3

-two

Question 4

Nutrient gelatin=

Answer 4

-medium composed of gelatin, peptone, and beef extract

Question 5

Gelatinase + organism=
Gelatinase - organism=

Answer 5

-liquifies the medium
-does not liquify the medium

Question 6

How long should the test tubes be incubated?

Answer 6

• 7 days

Question 7

The nutrient gelatin melts at >\?? therefore incubation is at ??

Answer 7

-28C
-25C

Question 8

The procedure uses a ?? because the agar is solid

Answer 8

-needle

Question 9

Gelatinase test:

Answer 9

-used to determine the ability of the microbe to produce gelatinases

Question 10

What is the primary purpose of nutrient gelatin medium in microbiology?

Answer 10

-to assess the ability of microorganisms to hydrolyze gelatin

Question 11

A gelatinase-positive organisms will:

Answer 11

-liquified the gelatin

Question 12

Gelatin is a source of:

Answer 12

-amino acids

Question 13

Gelatinases function to:

Answer 13

-Hydrolyze gelatin into amino acids.

Question 14

Which enzyme is commonly involved in the hydrolysis of gelatin in the nutrient gelatin test?

Answer 14

-gelatinase

Question 15

Which bacterial genera commonly produce gelatinase and give a positive result in the nutrient gelatin test?

==

Answer 15

-bacillus

Question 16

How long must the incubation period in the Gelatin Test should be:

Answer 16

-7 days