5.13: Casein Hydrolysis

Question 1

Casease:

Answer 1

-proteolyti enzyme that hydrolyses the milk protein casein (gives milk its white color)

Question 2

When casease is hydrolyzed the white color loses its ?? and becomes ??

Answer 2

-opacity
-clear

Question 3

Milk agar contains ??(4) which is used to detect the presence of casease

Answer 3

1) casein
2) yeast extract
3)dextrose
4)powdered milk

Question 4

Casease positive organism:
Casease negative organism:

Answer 4

-creates a zone of clearing where casein has been hydrolyzed
-do not secrete casease and do not produce clear zones around the growth

Question 5

The casease test is for identified organisms that use:

Answer 5

-Amino acids as an energy source

Question 6

A positive result for a casein hydrolysis test performed on milk agar plate will appear:

Answer 6

-as a clear halo

Question 7

The enzyme casease functions to

Answer 7

-hydrolyze casein

Question 8

What is the optimal temperature for performing the milk agar test?

Answer 8

-incubation at 37C

Question 9

What is the primary purpose of the milk agar test in microbiology?

Answer 9

-To assess the ability of microorganisms to hydrolyze casein

Question 10

The media used to test the presence of casease is:

Answer 10

-milk agar