Lab 1.5: Streak Plate Methods of Isolation Flashcards
Mixed cultureL
-a microbial culture consisting of 2 or more species
Pure culture:
-contains only a single species
What is the first step in identifying an organism?
-isolation of individual species from a mixed sample
What is a common isolation technique?
-streak plate
colonies
-cells consisting only of the original cell type
Colony forming unit (CFU):
-colonies form from individual cells and others from pairs, chains, or cluster of cells
The streak plate method produces individual colonies on an ?? plate
-agar
The inoculating loop should be transferred at an ?? angle
-45 degree
When performing an inoculation of a bacterium on a plate using the T-streak pattern, you must flame your inoculating loop ________ times before you flame your tool one last time when you have finished your work
3
The purpose of a streak plate is to:
-isolate a pure culture of the target organism
TRUE OR FALSE:
During streaking, the cell density of a microorganism decreases, leading to confluent growth.
-false
-confluent growth=uniform growth, no individual colonies
When performing an inoculation of a bacterium on a plate using the quadrant streak pattern, you must flame your inoculating loop ________ times before you flame your tool one last time when you have finished your work
four
When performing the streak plate method of inoculation you will need to use the
-inoculating loop
TRUE OR FALSE:
When performing an inoculation of a bacterium on a plate you must pressed your inoculating loop very hard against the solid medium.
-false
A commonly used isolation technique is the
-streak plate
