Lab 3.1-3.3- microscopy

Question 1

Father of the microscope:

Answer 1

-Antone Van Leewenhoek

Question 2

His single lens could only magnify a microbe ??X

Answer 2

-300X

Question 3

A simple microscope only has ?? lens

Answer 3

-one

Question 4

What kind of microscope is used in introductory classes and clinical laboratories?

Answer 4

-light microscopy
-specifically a compound light microscopy

Question 5

Bright field microscopy produces an image made from ?? that is transmitted through a specimen

Answer 5

-light

Question 6

Ocular lens:

Answer 6

-eyepiece
-magnifies the image formed by the objective lens

Question 7

Objective lens:-

Answer 7

-primary lenses that magnify the specimen

Question 8

Stage:

Answer 8

-holds the microscope slide in position

Question 9

condenser:

Answer 9

-focuses light through the specimen

Question 10

Diaphragm:

Answer 10

-controls the amount of light entering the condenser

Question 11

Illuminator:

Answer 11

-light source

Question 12

Light from the source is focused on the specimen by the ?? lens.

Answer 12

-condenser

Question 13

It then enter the ?? lens, where it is used to produced a magnified real image.

Answer 13

-objective

Question 14

Refraction:

Answer 14

-bending of light

Question 15

The real image is magnified again by ?? lens to produce a virtual image that is seen by the eye.

Answer 15

-ocular

Question 16

Magnification of each lens in a light compound microscope:

Answer 16

-4X
-10X
-40X
-100X

Question 17

By focusing the light, the condenser produces a…

Answer 17

-bright field illumination

Question 18

How should you carry a microscope?

Answer 18

-using both hands
-one hand holding the base and the other holding the arm

Question 19

What is usually used to gently clean the condenser and objective lens?

Answer 19

-lens paper

Question 20

To clean an ocular lens, moisten a cotton swab with ?? and gently wipe in a spiral motion.

Answer 20

-cleaning solution
-follow with dry swab

Question 21

When putting the microscope back in storage what magnifying lens position should it be in?

Answer 21

-4X

Question 22

What should you do with a plate of bacteria that will NOT be used anymore?

Answer 22

-place it in appropriate receptacle for autoclaving

Question 23

Autoclaving

Answer 23

-sterilization process that uses pressurized steam to kill infectious agents and denature proteins

Question 24

T OR F:
controls are not important when conducting an experiment

Answer 24

-False

Question 25

What should you do if you need to leave the laboratory temporarily in the middle of your work?

Answer 25

-remove your lab coat and gloves

Question 26

Media, cultures, equipment, and trash in a microbiological lab are sterilized using an —

Answer 26

-autoclave

Question 27

What is the primary purpose of keeping doors and windows closed during the lab session?

Answer 27

-to reduce the incidence of contamination from microbes traveling in the air

Question 28

Biosafety level 1:

Answer 28

-organisms do not typically cause disease in healthy individuals

Question 29

Biosafety level 2:

Answer 29

-organisms are commonly encountered in the community and present moderate hazard only

Question 30

Biosafety level 3:

Answer 30

-organisms are local or exotic and are associated with respiratory transmission and serious to lethal diseases

Question 31

Biosafety level 4:

Answer 31

-organisms have a great potential for lethal infection

Question 32

What protective clothing is recommended in a laboratory setting?

Answer 32

-long pants and closed toe shoes

Question 33

When should lab bench tops be cleaned with a disinfectant solution?

Answer 33

-at the beginning and end of the lab session