Lab 3.1-3.3- microscopy

Question 1

Father of the microscope:

Answer 1

-Antone Van Leewenhoek

Question 2

His single lens could only magnify a microbe ??X

Answer 2

-300X

Question 3

A simple microscope only has ?? lens

Answer 3

-one

Question 4

What kind of microscope is used in introductory classes and clinical laboratories?

Answer 4

-light microscopy
-specifically a compound light microscopy

Question 5

Bright field microscopy produces an image made from ?? that is transmitted through a specimen

Answer 5

-light

Question 6

Ocular lens:

Answer 6

-eyepiece
-magnifies the image formed by the objective lens

Question 7

Objective lens:-

Answer 7

-primary lenses that magnify the specimen

Question 8

Stage:

Answer 8

-holds the microscope slide in position

Question 9

condenser:

Answer 9

-focuses light through the specimen

Question 10

Diaphragm:

Answer 10

-controls the amount of light entering the condenser

Question 11

Illuminator:

Answer 11

-light source

Question 12

Light from the source is focused on the specimen by the ?? lens.

Answer 12

-condenser

Question 13

It then enter the ?? lens, where it is used to produced a magnified real image.

Answer 13

-objective

Question 14

Refraction:

Answer 14

-bending of light

Question 15

The real image is magnified again by ?? lens to produce a virtual image that is seen by the eye.

Answer 15

-ocular

Question 16

Magnification of each lens in a light compound microscope:

Answer 16

-4X
-10X
-40X
-100X

Question 17

By focusing the light, the condenser produces a…

Answer 17

-bright field illumination

Question 18

How should you carry a microscope?

Answer 18

-using both hands
-one hand holding the base and the other holding the arm

Question 19

What is usually used to gently clean the condenser and objective lens?

Answer 19

-lens paper

Question 20

To clean an ocular lens, moisten a cotton swab with ?? and gently wipe in a spiral motion.

Answer 20

-cleaning solution
-follow with dry swab

Question 21

When putting the microscope back in storage what magnifying lens position should it be in?

Answer 21

-4X

Question 22

What should you do with a plate of bacteria that will NOT be used anymore?

Answer 22

-place it in appropriate receptacle for autoclaving

Question 23

Autoclaving

Answer 23

-sterilization process that uses pressurized steam to kill infectious agents and denature proteins

Question 24

T OR F:
controls are not important when conducting an experiment

Answer 24

-False

Question 25

What should you do if you need to leave the laboratory temporarily in the middle of your work?

Answer 25

-remove your lab coat and gloves

Question 26

Media, cultures, equipment, and trash in a microbiological lab are sterilized using an —

Answer 26

-autoclave

Question 27

What is the primary purpose of keeping doors and windows closed during the lab session?

Answer 27

-to reduce the incidence of contamination from microbes traveling in the air

Question 28

Biosafety level 1:

Answer 28

-organisms do not typically cause disease in healthy individuals

Question 29

Biosafety level 2:

Answer 29

-organisms are commonly encountered in the community and present moderate hazard only

Question 30

Biosafety level 3:

Answer 30

-organisms are local or exotic and are associated with respiratory transmission and serious to lethal diseases

Question 31

Biosafety level 4:

Answer 31

-organisms have a great potential for lethal infection

Question 32

What protective clothing is recommended in a laboratory setting?

Answer 32

-long pants and closed toe shoes

Question 33

When should lab bench tops be cleaned with a disinfectant solution?

Answer 33

-at the beginning and end of the lab session

Question 34

select all features found in light microscope:
-use magnifying lenses with visible light
-resolve structures that are .2 nm apart
-use beam of electrons
-resolve structures that are 200 nm apart

Answer 34

-use magnifying lenses with visible light
-resolve structures that are 200 nm apart

Question 35

When comparing prokaryotic cells and eukaryotic cells, prokaryotes are —- and —- than eukaryotes.

Answer 35

-smaller and simpler

Question 36

In order to visualize a virus particle you will need to use a —- microscope.

Answer 36

-electron

Question 37

The total magnification achieved by the microscope is calculated by — the magnification of the objective and the ocular lens.

Answer 37

-multiplying

Question 38

TRUE OR FALSE:
The factor that most limits the clarity of the image in a microscope is the magnification.

Answer 38

False

Question 39

Why is it important to center the object you are analyzing in the field of view of the microscope before switching to a higher power lens?

Answer 39

-as magnification increases, field diameter decreases, and objects at the periphery will no longer be in the visible field.