Lab 3.1-3.3- microscopy Flashcards

1
Q

Father of the microscope:

A

-Antone Van Leewenhoek

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2
Q

His single lens could only magnify a microbe ??X

A

-300X

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3
Q

A simple microscope only has ?? lens

A

-one

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4
Q

What kind of microscope is used in introductory classes and clinical laboratories?

A

-light microscopy
-specifically a compound light microscopy

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5
Q

Bright field microscopy produces an image made from ?? that is transmitted through a specimen

A

-light

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6
Q

Ocular lens:

A

-eyepiece
-magnifies the image formed by the objective lens

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7
Q

Objective lens:-

A

-primary lenses that magnify the specimen

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8
Q

Stage:

A

-holds the microscope slide in position

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9
Q

condenser:

A

-focuses light through the specimen

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10
Q

Diaphragm:

A

-controls the amount of light entering the condenser

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11
Q

Illuminator:

A

-light source

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12
Q

Light from the source is focused on the specimen by the ?? lens.

A

-condenser

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13
Q

It then enter the ?? lens, where it is used to produced a magnified real image.

A

-objective

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14
Q

Refraction:

A

-bending of light

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15
Q

The real image is magnified again by ?? lens to produce a virtual image that is seen by the eye.

A

-ocular

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16
Q

Magnification of each lens in a light compound microscope:

A

-4X
-10X
-40X
-100X

17
Q

By focusing the light, the condenser produces a…

A

-bright field illumination

18
Q

How should you carry a microscope?

A

-using both hands
-one hand holding the base and the other holding the arm

19
Q

What is usually used to gently clean the condenser and objective lens?

A

-lens paper

20
Q

To clean an ocular lens, moisten a cotton swab with ?? and gently wipe in a spiral motion.

A

-cleaning solution
-follow with dry swab

21
Q

When putting the microscope back in storage what magnifying lens position should it be in?

22
Q

What should you do with a plate of bacteria that will NOT be used anymore?

A

-place it in appropriate receptacle for autoclaving

23
Q

Autoclaving

A

-sterilization process that uses pressurized steam to kill infectious agents and denature proteins

24
Q

T OR F:
controls are not important when conducting an experiment

25
What should you do if you need to leave the laboratory temporarily in the middle of your work?
-remove your lab coat and gloves
26
Media, cultures, equipment, and trash in a microbiological lab are sterilized using an ---
-autoclave
27
What is the primary purpose of keeping doors and windows closed during the lab session?
-to reduce the incidence of contamination from microbes traveling in the air
28
Biosafety level 1:
-organisms do not typically cause disease in healthy individuals
29
Biosafety level 2:
-organisms are commonly encountered in the community and present moderate hazard only
30
Biosafety level 3:
-organisms are local or exotic and are associated with respiratory transmission and serious to lethal diseases
31
Biosafety level 4:
-organisms have a great potential for lethal infection
32
What protective clothing is recommended in a laboratory setting?
-long pants and closed toe shoes
33
When should lab bench tops be cleaned with a disinfectant solution?
-at the beginning and end of the lab session
34
select all features found in light microscope: -use magnifying lenses with visible light -resolve structures that are .2 nm apart -use beam of electrons -resolve structures that are 200 nm apart
-use magnifying lenses with visible light -resolve structures that are 200 nm apart
35
When comparing prokaryotic cells and eukaryotic cells, prokaryotes are —- and —- than eukaryotes.
-smaller and simpler
36
In order to visualize a virus particle you will need to use a —- microscope.
-electron
37
The total magnification achieved by the microscope is calculated by — the magnification of the objective and the ocular lens.
-multiplying
38
TRUE OR FALSE: The factor that most limits the clarity of the image in a microscope is the magnification.
False
39
Why is it important to center the object you are analyzing in the field of view of the microscope before switching to a higher power lens?
-as magnification increases, field diameter decreases, and objects at the periphery will no longer be in the visible field.