5.1: Starch Hydrolysis Flashcards
Hydrolysis reaction:
-reactions that use water to split complex molecules
the enzymes required for a hydrolysis reactions are called:
-hydrolytic enzymes
Starch is a:
-polysaccharide, carb
Nutrient gelatin detects ??
-gelatinase
-an exoenzyme that liquifies the solid medium
Exoenzyme
-enzymes secreted from the organism to catalyze reactions outside the cell
Starch is a complex polysaccharide composed of alpha glucose polymers in one of two forms:
-linear (amylose)
-branched (amylopectin)
Starch is too ?? to pass through the bacterial cell membrane:
-large
three enzymes involved in starch digestion:
-alpha amylase: break it into glucose and maltose
-beta amylase: produces maltose
-alpha 1,6 glucosidase
Starch agar:
-beef extract, soluble starch, and agar
If the organism produces the 3 enzymes they will hydrolyze the starch and produce a:
-clear halo around their growth
Because starch and the sugar subunits are virtually invisible in the medium, the reagent ?? is used as a color indicator of growth
-iodine
-reacts with starch to produce a blue/dark brown color
The enzymes required for an organism to hydrolyze the glucose polysaccharide starch, are oligo-1,6-glucosidease and:
-amylose
What is the purpose of the starch agar test in microbiology?
-to asses the ability of microorganisms to hydrolyze starch
The α-amylase, β-amylase and α-1,6-glucosidase are:
-exoenzymes
The function of the iodine in the starch hydrolysis test is to:
-make starch visible
