5.2 Phenol Red Fermentation Broth Flashcards
Which of the following carbohydrates is commonly used in the Phenol Red Fermentation Broth test?
-all of the above (glucose, lactose, sucrose)
Following incubation of an organism in a tube of phenol red glucose broth, you observe the tube is pink with no visible bubbles in the Durham tube. What can you reasonably conclude about this organism?
-It deaminate the amino acids producing ammonia (NH3).
When the Phenol Red Fermentation Broth turns yellow, this indicates:
-an acidic pH
The phenol red in the Phenol Red Fermentation Broth functions:
-as a pH indicator
When the Phenol Red Fermentation Broth turns pink, this indicates:
-a basic pH
What is the primary endpoint of the Phenol Red Fermentation Broth test?
-pH change
The Phenol Red Fermentation Broth test is used for?
-Identified if the organisms ferment carbohydrates
What is the purpose of the inclusion of a Durham tube in differential culture media?
-To visualize the production of gas during growth.
-also form fermentation
What is the clinical significance of the Phenol Red Fermentation Broth test?
-It assists in differentiating bacteria based on their carbohydrate fermentation abilities.
Following incubation of an organism in a tube of phenol red glucose broth, you observe the tube to be red with no visible bubbles in the Durham tube. What can you reasonably conclude about this organism?
-It does not ferment glucose.
Following incubation of an organism in a tube of phenol red glucose broth, you observe the tube to be yellow with a bubble in the Durham tube. What can you reasonably conclude about this organism?
-It ferments glucose to both acid and gas products.
Deamination of amino acids supplied
by casein (milk protein) results in an
alkaline reaction from the ammonia
(NH3) that is produced. This raises the pH and turns the broth ??
-pink
