5.4: Catalase Test Flashcards
Catalse:
-enzyme that detoxifies the cell
-test will show if organism can
in respiration, the energy from glucose is transferred into high energy bonds ?? using glycolysis, the citric acid cycle, and oxidative phosphorylation in the ??
-ATP
-electron transport chain
The final electron acceptor in all respiratory pathways is an ??
-inorganic molecule
The catalase test demonstrates the presence of an ??, by the organisms ability to produce ??, which is an enzyme that detoxifies the cell by converting hydrogen peroxide produce in the ETC to H2O and O2
-ETC
-catalase
Hydrogen peroxide and superoxide radicals are toxic because…
-they oxidize biochemicals and make them non-functional
organisms that produce them also produce enzymes capable of :
-breaking them down
Catalase is an enzyme of ??, ?? and ?? that converts hydrogen peroxide to water and oxygen gas and allows them to grow in the presence of oxygen
-aerobes
-microaerophiles
-facultative anaerobes
Bubbles=
No bubbles=
-catalase positive
-catalase negative
A negative result in the catalase test is indicated by
-No bubbles upon addition of hydrogen peroxide.
Catalase is an enzyme that is not present in
-anaerobes
A positive result in the catalase test is indicated by
-Bubbles upon addition of hydrogen peroxide.
TRUE OR FALSE:
For the catalase test, you will first add catalase and then transfer a visible amount of bacterial growth to a glass slide
-false
Superoxide dismutase is an enzyme that functions to
-Convert superoxide radicals into hydrogen peroxide.
TRUE OR FALSE:
Hydrogen peroxide is a highly potent cellular toxin.
-true
TRUE OR FALSE:
In large part, the ability of a microorganism to synthezise the protective enzyme gelatinase accounts for that organism’s ability to live in the presence of oxygen.
-FASLE
