Lab 1

Question 1

What is the function of the eyepiece?

Answer 1

connects lens to the body and allows proper alignment of light travel

Question 2

What is the magnification of the ocular lens?

Answer 2

10x

Question 3

What is the function of the ocular lens?

Answer 3

enhances details of the specimen and magnifies image

Question 4

What is the 4x lens called?

Answer 4

Scanning lens

Question 5

What is the 4x lens used for?

Answer 5

quickly surveying a sample

Question 6

What is the 10x lens called?

Answer 6

Low power lens

Question 7

What is the 10x lens used for?

Answer 7

Ideal for observing larger specimen details

Question 8

What is the 40x lens called?

Answer 8

High power lens

Question 9

What is the 40x lens used for?

Answer 9

examining fine details of a specimen

Question 10

What is the 100x lens called?

Answer 10

Oil Immersion Lens

Question 11

What is the 100x lens used for?

Answer 11

enhancing resolution with oil

Question 12

What is the function of the objective lens?

Answer 12

gathers light from specimen and helps with magnifying image

Question 13

How should order of objective lens be looked at?

Answer 13

4x to 10x to 40x to 100x

Question 14

How do you change objective lens?

Answer 14

use the revolving nose piece

Question 15

How do you clean oil immersion lens?

Answer 15

with lens paper and a little alcohol

Question 16

How do you calculate total magnification?

Answer 16

objective lens x ocular lens

Question 17

What is the function of the stage?

Answer 17

holds the slide

Question 18

What is the function of the slide adjuster knob?

Answer 18

moves slides without touching it

Question 19

What does the coarse focus knob do?

Answer 19

use for 4x and 10x
- brings specimen into general focus

Question 20

What does fine focus knob do?

Answer 20

sharpens image and clarity

Question 21

What is the relationship between magnification and visual field?

Answer 21

Inversely proportional
- decrease magnification = increase in visual field
- increase magnification = decrease in visual field

Question 22

What is the relationship between magnification and depth of field?

Answer 22

Inversely proportional
- increase in magnification = decrease in depth
- decrease in magnification = increase in depth

Question 23

What is the function of the condenser lens?

Answer 23

regulates brightness of light

Question 24

What is the bright field setting good for?

Answer 24

dead bacteria
- light passes through
- colored specimens

Question 25

What is the dark field setting good for?

Answer 25

live, unstained specimens
- light reflects off

Question 26

What is the function of the iris diaphragm?

Answer 26

adjusts light intensity by changing size of aperture
- like the iris

Question 27

How do you handle the microscope?

Answer 27

• 2 hands (one on arm, one on base)
• completely on table top
• take off cover and put it back in the cabinet
• plug in and turn on

Question 28

How do you put away the microscope?

Answer 28

• turn off
• wrap cord nicely
• lower and center stage
• light to lowest setting
• 4x lens on
• put into cabinet with ocular lens facing inside