Lab 1 Flashcards

1
Q

What is the function of the eyepiece?

A

connects lens to the body and allows proper alignment of light travel

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2
Q

What is the magnification of the ocular lens?

A

10x

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3
Q

What is the function of the ocular lens?

A

enhances details of the specimen and magnifies image

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4
Q

What is the 4x lens called?

A

Scanning lens

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5
Q

What is the 4x lens used for?

A

quickly surveying a sample

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6
Q

What is the 10x lens called?

A

Low power lens

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7
Q

What is the 10x lens used for?

A

Ideal for observing larger specimen details

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8
Q

What is the 40x lens called?

A

High power lens

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9
Q

What is the 40x lens used for?

A

examining fine details of a specimen

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10
Q

What is the 100x lens called?

A

Oil Immersion Lens

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11
Q

What is the 100x lens used for?

A

enhancing resolution with oil

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12
Q

What is the function of the objective lens?

A

gathers light from specimen and helps with magnifying image

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13
Q

How should order of objective lens be looked at?

A

4x to 10x to 40x to 100x

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14
Q

How do you change objective lens?

A

use the revolving nose piece

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15
Q

How do you clean oil immersion lens?

A

with lens paper and a little alcohol

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16
Q

How do you calculate total magnification?

A

objective lens x ocular lens

17
Q

What is the function of the stage?

A

holds the slide

18
Q

What is the function of the slide adjuster knob?

A

moves slides without touching it

19
Q

What does the coarse focus knob do?

A

use for 4x and 10x
- brings specimen into general focus

20
Q

What does fine focus knob do?

A

sharpens image and clarity

21
Q

What is the relationship between magnification and visual field?

A

Inversely proportional
- decrease magnification = increase in visual field
- increase magnification = decrease in visual field

22
Q

What is the relationship between magnification and depth of field?

A

Inversely proportional
- increase in magnification = decrease in depth
- decrease in magnification = increase in depth

23
Q

What is the function of the condenser lens?

A

regulates brightness of light

24
Q

What is the bright field setting good for?

A

dead bacteria
- light passes through
- colored specimens

25
What is the dark field setting good for?
live, unstained specimens - light reflects off
26
What is the function of the iris diaphragm?
adjusts light intensity by changing size of aperture - like the iris
27
How do you handle the microscope?
- 2 hands (one on arm, one on base) - completely on table top - take off cover and put it back in the cabinet - plug in and turn on
28
How do you put away the microscope?
- turn off - wrap cord nicely - lower and center stage - light to lowest setting - 4x lens on - put into cabinet with ocular lens facing inside