Human genome prodject

Question 1

What did scientists do after the structure of DNA was discovered

Answer 1

They developed a method of sequencing the DNA

Question 2

What is DNA sequencing

Answer 2

The process used to determine the precise sequence of nucleotides in a length of DNA

Question 3

Who was the pioneer of DNA sequencing

Answer 3

Fred Sanger who developed the Sanger sequencing method

Question 4

1st step of Sanger sequencing

Answer 4

Synthesis of many DNA fragments that differ in length by one base pair from a DNA template strand

Question 5

2nd step of Sanger sequencing

Answer 5

The fragments are then separated out according to length using gel electrophoresis and the end base is identified

Question 6

What does Sanger sequencing allow

Answer 6

The base sequence of the DNA strand to be worked out

Question 7

How was DNA sequencing used

Answer 7

Enabled the launch of the human genome project which aimed to sequence the entire human genome

Question 8

What were human genome projects aims

Answer 8

• The determination of the entire base sequence of genomes from simpler eukaryotic organisms
• The determination of the entire base sequence of the human genome
• Identification of new genes and determination of their location, function and regulation
• The discussion of ethical, legal and social issues that genome sequencing creates