Histology: Diversity of Cells and Their Functions Flashcards
how is a tissue fixed and preserved
a chemical that cross-links proteins i.e. formalin is used
describe the process of creating a tissue slide
- tissue is thinly sliced
- tissue dehydrated and put in organic solvent and hot wax
- microtome cuts thin slices
- tissue put on slides, wax is washed out, tissue rehydrated and stains are put in
what are artefacts
distortions/changes from original tissue
what are the most common stains
Haematoxylin and Eosin (H and E)
Haematoxylin is _ and stains _ molecule turning them _
basic, acidic, blueish-purple
Eosin is _ and stains _ molecules turning them _
acidic, basic, pinkish-red
what usually stains with eosin
cytoplasm
what are the 4 basic tissue types
- epithelium
- muscle
- connective
- nervous
what do epithelial cells do
cover body surfaces/line hollow organs and form many glands
what are the roles of connective tissue
- development, growth and homeostasis of tissues
- energy storage (i.e. fat)
what is the role of muscle cells
generate force by contracting
what does nervous tissue consist of
neurons and supporting cells
what is the role of nervous tissue
control function and allow rapid communication wihtin the body
what is a basal lamina
layer of extracellular matrix components that epithelial cells are attached to
are epithelial cells vascular or non-vascular
non-vascular
where do epithelial cells get their nutrients
from capillaries in underlying tissues - nutrients diffuse across basal lamina
what are the functions of epithelial cells (8)
- chemical barrier
- locomotion
- absorption
- secretion.
- mechanical barroer
- containment
minor - sensation and contractility
what are the 3 epithelial cell shapes
- columnar
- squamous
- cuboidal
how many layers of epithelial cells in a:
- simple
- stratified
- pseudostratified epithelium
- simple - 1
- stratified - 2+
- pseudostratified - looks like multiple but all cells are in contact with basal lamina
what are the 3 specialisations of epithelium
- keratinised
- villi
- microvilli (brush border)
what specialised cell may appear in epithelium and what is its function
- goblet cell - single cell, mucous gland
what does glandular epithelium do
produce secretions i.e. milk, mucous, hormones, enzymes, oil
describe an endocrine gland
- product secreted towards basal end of the cell
- ductless
- product distributed in vascular system