Biochemistry: Enzyme Kinetics Flashcards
what changes can result in a major implications for disease in terms of enzymes
distribution, abundance, efficiency
what can enzymes be used for in the lab
to develop diagnostic tests and drugs
in terms of enzymes activity, what is V
the no. of moles of a substrate converted per second
in terms of enzymes activity, what is Vmax
the maximum rate of enzyme activity (at infinite [S])
in terms of enzymes activity what is, what is half Vmax
the half-maximal rate of enzyme activity
draw the equation showing enzyme activity
E + S -K+1-> ES -K+2-> E + P
how do you measure Vmax
measure the initial reaction velocity at a known [S] and then increase [S] recording the reaction rate
what is the Michaelis constant
Km - the [S] at which the rate 1/2Vmax
does reaction rate ever reach Vmax
no
how would you calculate Km and Vmax for a true result
convert the results onto a straight line graph
what does a low Km mean
little substrate is needed to ilicit a have Vmax response
what does a high Km mean
a lot of substrate is needed to ilicit a have Vmax response
give an example of a reaction with a low Km and why
- energy production in RBCs
- maintains the reaction even if there is a low glucose conc.
give an example of a reaction with ah igh Km and why
- Proline Hydoxylases, need O2 to regulate transcription factors
- allows O2 sensitive O2 sensing over physiological O2 ranges
what disease can a loss of proline hydroxylase result in
Monge’s Disease +/- Von Hippel Lindaw Syndrome
