GEN BIO: RESEARCH PROCEDURES Flashcards
Light microscope
-Light is first passed through a specimen and then through a glass lens, which bends light in such a manner that an image is magnified
-many internal structures of the cell are too small to be seen, even through the light microscope, and thus the invention of the electron microscope
Electron microscope
-invented in the 1950s greatly enhanced the study of cell biology
-sends a beam of electrons through a specimen
-can be used to examine structures that are too small to be seen through the light microscope
-the light microscope can magnify specimens up to 2000x whereas the electron microscope can magnify up to 2 million times
Compound Microscope
-Magnify approximately 400 times under high power
-quality of lens determines resolution of microscope
-often stain the specimen before to make particular structures stand out
-common stains include Lugol’s solution, methylene blue, and Wright’s stain
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Variable
-Measurable factors or qualities that change during an experiment
-classified as independent and dependent
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Independent variable
One that is changed by the experimenter
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Dependent variable
Changes in response to the independent variable
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Constants
Factors that do not change
Controlled experiment
-Seeking data on only one independent variable
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Representing in the form of a graph
The independent variable is always plotted on the x-axis and the dependent variable on the y-axis