Final Flashcards
identify the building blocks of DNA
DNA (Deoxyribonucleic acid) is made up of nucleotides
nucleotides have three parts: a sugar, a phosphate group, and a nitrogenous base
describe DNA’s structure and the rules for base pairing
DNA is in a form of a double helix=two strands of DNA twist together with the sugar-phosphate backbone on the outside and nitrogenous base on the inside. (nitrogenous pairs can only pair: adenine–>thymine and guanine–>cytosine)
explain how the template mechanism is important in DNA replication
the template is important in DNA replication because it allows for a complimentary strand to be made (after the two double helix strands separated) so nucleotides can be added and create a new DNA strand
describe the process of DNA replication. explain the “one gene-one polypeptide” hypothesis
- DNA replication is the process of copying the DNA molecule by separating the two strands of the double helix, they use the separated strands as a template called the complimentary strand in order for nucleotides to be added and so DNA polymerase can form bonds and create a new DNA strand
- states that each gene controls the production of a specific polypeptide (scientists know most genes code for the production of a single polypeptide)
trace the information flow from DNA to protein
first, messenger RNA is transcribed from the DNA molecule. an enzyme called RNA polymerase joins the RNA nucleotides together during transcription. RNA splicing removed introns and joins the exams. then it is transferred to the cytoplasm. transfer RNA binds to the mRNA. the process of bringing amino acid into position on the ribosome (ribosomal RNA)
describe how amino acids are coded
transfer RNA (has an amino acid binding site at one end) binds to the mRNA bringing an amino acid into position to be added to the polypeptide (takes place on a ribosome=ribosomal rna)
describe the process of DNA transcription
the DNA sequence is converted to the form of a single strand RNA molecule
describe how RNA is translated to a protein
in the cytoplasm translation converts the nucleic acid sequence into a sequence of amino acids
summarize protein synthesis
the DNA is transcribed in the nucleus, the introns are removed, the exams enter the cytoplasm as mRNA (by translation) the mRNA binds with the tRNA on a ribosome (rRNA)
describe the types of mutations that can affect genes
mutations=a change in a single nucleotide or a change in a large part of a chromosome
- substitution:one nucleotide is replaced by another
- insertion/deletion: one or more nucleotides are added to/removed from a gene
explain what can cause a mutation
errors in crossing over or DNA replication and also a mutagen (physical/chemical agent that causes a mutation)
describe how DNA is packs within the nucleus
first, DNA molecule wraps tightly around small proteins called histones. next, the DNA and the histones wrap into a tight cool. the coil twists again into a thick structure called a “supercoil”
describe the significance of the human genome project
medical researchers use the data to identify genes associated with diseases and to develop treatment for genetic disorders
relate down syndrome and nonseperation/nondisjunction of chromosomes
trisomy 21 which is when a person has three #21 chromosomes. I normally happens because of nondisjunction which is when a pair of homologous chromosomes or sister chromatids fail to separate during meiosis
describe how chromosomes can be damaged
nondisjunction: pair of homologous chomosomes or sister chromatids that fail to seperate
duplication: occurs when a part of chromosome is repeated
deletion: occurs when a part of a chromosome is lost
inversion: occurs when part of a chromosome is reversed
translocation: occurs when a portion of a chromosome attaches to a non-homologous chromosome
summarize the information provided in a pedigree
used to study the inheritance of traits in a human by tracing the occurence of a trait in a family
explain how recessive, dominant, and sex-linked disorders are inherited
recessive: need both alleles to have the disorder, if you have one you are a carrier and none you don’t have it at all
dominant: only need one allele for the disorder to show, if you have none it won’t show
sex-linked: the allele for the trait is located on the X chromosome. males are more at risk than females
describe how it is possible to predict certain genetic disorders
a genetic counselor is a person who is trained to collect and interpret family histories of genetic disorders
Explain how mutations to genes that play a role in regulating the cell cycle can lead to cancer
a mutation can cause a gene to be cancer-causing (oncogene)
explain how the use of bacteria has contributed to the development of DNA technology
biotechnology: involves changing the genomes of organisms using DNA technology
list some of the recent research trends in recombinant DNA technology
medicine, agriculture, and other fields
explain the role of plasmids in engineering bacteria
they use plasmids to move genes into bacteria
explain how biologists “cut and paste” DNA
a restriction enzyme “cuts” a dna molecule into fragments at specific points. Another enzyme “pastes” a fragment carrying a particular gene into a plasmid
describe the procedure used in cloning a specific gene
a restriction enzyme “cuts” a dna molecule into fragments at specific points. Another enzyme “pastes” a fragment carrying a particular gene into a plasmid. The plasmid is put back into a bacterial cell which then reproduces and creates a clone