EX 1- Staphylococcus spp.- Daniels

Question 1

Staphylococcus is gram _________

Answer 1

positive

Question 2

Staphylococcus is non ____, non ___ ____ and predominantly _______ _______

Answer 2

motile, spore forming, faculatative anaerobes

Question 3

What test is used to identify an infection as staph?

Answer 3

catalase test (positive produces bubbles)

Question 4

What test is used to differentiate staphylococcus species?

Answer 4

Coagulase test

Question 5

What is a coagulase positive staphylococci that causes the most burden in people?

Answer 5

S. Aureus

Question 6

What conditions is S. Aureus the causative agent?

Answer 6

• Aspiration pneumonia
• UTI
• Toxic Shock Syndrome
• Scalded Skin Syndrome
• Food poisoning

Question 7

S. Aureus lives on and around _____, 30-40 % prevalence in the ____ ____

Answer 7

people, nasal carriage

Question 8

What are the two ways that S. Aureus enters the body?

Answer 8

• Damage to skin/follicles (wounds, burns, bites)

- Damage to mucosal surfaces

Question 9

What does the spread of S. Aureus in the body depend upon?

Answer 9

• Bacterial inoculum (more worse)
• Host immunocompetence
• Location of infection

Question 10

Bacteremia + _____ compromise = trouble

Answer 10

immune

Question 11

What populations are at elevated risk for S. Aureus?

Answer 11

Immuno-compromised patients

• young children/elderly
• diabetics
• ppl on immunosuppresive therapy
• HIV+
• dialysis patients
• IV drug users

Question 12

What factors enhance the ability of S. Aureus to colonize and invade a host?

Answer 12

MSCRAMMs

• Fibronectin binding proteins
• Collagen binding protein (CNA)
• clumping factors (bind fibrinogen)