E3: L20 Discussion Flashcards
On a histogram concerning a flow cytometry run, the Y axis shows _____ while the X axis shows the ____.
the number of events or cell count, the parameters digital signal value.
Why are multiple (12-14) stains used when using flow cytometry?
Multiple stains allow the observer to identify and separate the multiple cell types present due to each cell type absorbing a different wavelength.
Forward scatter is proportional to ______ as side scatter is to ______.
a. cell size, granularity
b. granularity, cell size
c. cell size, population size
d. population size, cell size
A
Which can be used in flow cytometry to allow analysis of only a specific cell type?
a. chain
b. gate
c. fence
d. wavelength
B
Which of the following best describes the speed capabilities of flow cytometry?
a) Slow, analyzing a few cells per minute
b) Moderate, analyzing hundreds of cells per minute
c) High, analyzing thousands to tens of thousands of cells per second
d) Variable, depending on the operator’s proficiency
C
What are the disadvantages of using flow cytometry?
lack of affordability
What does each dot on a ‘dot plot’ correspond to?
A. An individual being tested
B. A group of cells
C. An individual cell
D. None of these
C
What are the physical characteristics of cell populations that are dectected by cell sorting in flow cytometry?
A. Cell size, Cell shape, Cell count
B. Cell size, Cell morphology, Cell protein expression
C. Cell count, Cell morphology, Cell color
D. Cell protein expression and Cell count
B
What are the five uses of Flow Cytometry?
Cell Counting, Cell Sorting, Determining Cell Characteristics and Function, Detecting Microorganism, and Diagnosis of health disorders.
What does FACS mean?
Fluorescence Activation Cell Sorting
