DNA Synthesis

Question 1

The daughter DNA strand is synthesized in which direction?

Answer 1

5’–>3’

Question 2

The template strand of DNA is read in which direction during synthesis of a daughter strand?

Answer 2

3’–>5’

Question 3

DNA Pol requires what in order to replicate DNA?

Answer 3

Free 3’ –OH

Question 4

This enzyme adds primers to DNA for replication

Answer 4

Primase (RNA primer)

Question 5

Function of telemerase

Answer 5

Extend telomeres using reverse transcription

Question 6

Prokaryotic DNA replication initiation

Answer 6

Binding of DnaA protein at point of orgin OriC, proceeds bidirectionally

Question 7

This enzyme adds RNA primers to DNA strand in prokaryotic replication

Answer 7

DNA primase

Question 8

This DNA Pol fills in the gaps left by Okazaki fragments

Answer 8

Pol I

Question 9

Function of clamp proteins (prokaryotic)

Answer 9

Hold DNA Pol onto template strand

Question 10

DNA Pol that has “proofreading” function

Answer 10

DNA Pol III

Question 11

DNA synthesis can only occur during what time of the eukaryotic cell cycle?

Answer 11

S phase

Question 12

What is the major DNA Pol in eukaryotes?

Answer 12

Pol delta

Question 13

How long are Okazaki fragments in eukaryotes?

Answer 13

~200 bp

Question 14

Two functions of eukaryotic topoisomerases

Answer 14

1. Relieve tension (Topo I)

2. Untangle DNA (Topo II)–>cancer target, only in proliferating cells