DNA & RNA Chapter Test Flashcards
DNA
Deoxyribonucleic acid
Determined that DNA contains the genetic material
Hershey and Chase
Determined the shape of DNA to be a double helix
Watson and Crick; Rosalind Franklin
Monomer of DNA
Nucleotides
Made up of a sugar, a phosphate, and a base that is attached to the sugar
Nucleotides
Sugar and phosphate create the _____ to nucleotides
Backbone
Sugar in DNA
Deoxyribose
4 possible bases in DNA
Adenine
Guanine
Cytosine
Thymine
Double sided molecule that is twisted
DNA
When only certain bases will bind with other bases
Complementary base pairing
Function of DNA
Stores all genetic material for the cell
Where is DNA located?
Lives in the nucleus but can also be found in the mitochondria
Polymer of RNA
Nucleus acid
Monomer of RNA
Nucleotides
Sugar in RNA
Ribose
Bases in RNA
Adenine
Guanine
Cytosine
Uracil
Structure of RNA
Single-stranded (not twisted)
Types of RNA
rRNA
mRNA
tRNA
Structural component of the ribosome (protein synthesis)
Ribosomal RNA
Where is rRNA made?
Nucleolus
Carries the message from DNA in the nucleus to the ribosome
Messenger RNA
Brings amino acids to the ribosome
Transfer RNA
Cellular processes
DNA replication
Transcription
Translation
The copying of the genetic material
DNA replication
The copying of the DNA message by mRNA in the nucleus
Transcription
The construction of s protein at the ribosome by tRNA reading the mRNA and assembling amino acids
Translation
Where does DNA replication occur?
Nucleus
Every cell must have all of the _____ _____, but all cells don’t necessarily use all of it
Genetic information
How do cells get all of the genetic information?
Replication bubbles
The fact that in each of the copies of DNA there is one new strand and one old strand
Semi conservative replication
Enzymes in DNA replication
Helicase
DNA polymerase
DNA ligase
Unwinds the alpha-helical structure of the DNA
Helicase
In replication bubbles, it adds nucleotides to the new DNA strand
DNA polymerase
Binds the nucleotides to the DNA strand
DNA ligase
Where does transcription occur?
Nucleus
Polymer of transcription
Proteins
Monomer of transcription
Amino acids
Came up with the “one gene only codes for one polypeptide” idea
Beadle and Tatum
The goal of transcription is to make ______
Proteins
In transcription, DNA is copied by ____
mRNA
The enzyme ___ ______ catalyzes transcription
RNA polymerase
The mRNA strand is _____ in the ____
Modified; nucleus
DNA sequences that don’t code for proteins
Introns
Sequences of DNA that code for proteins
Exons
Original strand before it’s modified
pre-mRNA
What modifies the pre-mRNA?
snRNP
Small, nuclear ribonuclear proteins that cut out what pre-mRNA don’t need
snRNP
Where does pre-mRNA get modified?
Nucleus
mRNA is read in a series of ___
3
A series of 3 mRNA
Codon
Each 3-code of mRNA code for a different ____ _____
Amino acid
Building of a protein at the ribosome using the message from mRNA, tRNA is also involved
Translation
mRNA sends the ____ _____ to the ______ and tRNA sends _____ _____ to the _____ in translation
DNA message; ribosome; amino acids; ribosome
Made up of two subunits; site of protein synthesis
Ribosomes
Names of the subunits of ribosomes?
Large and small
How many tRNA binding sites are there?
2
Names of the tRNA binding sites
A and P
3-base sequence of tRNA
Anticodon
The ______ is the complement to the codon
Anticodon
List of what 3 bases code for what amino acid
Genetic dictionary
The start codon; only codes for methionine
AUG
Every protein begins with ______ but almost always gets it taken off
Methionine
Terminator codons
UAA, UGA, UAG
Any change in the nucleotide sequence; can involve a single nucleotide or a large region
Mutations
Single nucleotide mutation
Base substitution
One base is substituted for another accidentally; usually harmful
Base substitution
Effects of base substitution
Nothing
Causes a protein to be dysfunctional
Non-functional protein
Enhanced functional protein
Type of base substitution
Base insertion of deletion
Shifts the reading frame
Base insertion or deletion
Base insertion of deletion is usually __ _____
A disaster
Causes of base insertion of deletion
Spontaneous (accidental and random)
Physical (X-rays or UV light)
Chemical agents
Causes a mutation
Mutagen
The act of causing a mutation
Mutagenesis