DNA Repair and Replication Flashcards
what are the 3 fundamental condition of DNA replication?
semiconservative, bidirectional, semi discontinuous
in the semi conservative method, one strand of the newly synthesized strand is ____ to the original
complementary (A-T, C-G)
what is the name of the site of origin in unidirectional replication?
Ori-C
okazaki fragments are liked via ____ bonds
covalent
in what direction is the leading strand synthesized?
5’ to 3’
what enzyme synthesizes DNA?
DNA polymerase
____ ____ is the enzyme that links together okazaki fragments
DNA ligase
what are the 3 phases of DNA replication?
initiation, elongation, termination
what is the initiation factor that binds to OriC to separate the strands of the double helix?
DnaA protein
what is the role of DnaA protein?
bind to oriC to separate the strands of the double helix
what helicase binds to the separated strands and moves alone the DNA to unwind the DNA double helix?
DnaB protein
what is the role of DnaB protein?
bind to the separated strands to unwind the double helix
what is the protein that prevents the two unwound DNA strands from coming back together?
SSB (single-stranded DNA binding protein)
what is the role of SSB (single-strand DNA binding protein)?
prevent the separated strands from coming back togehter
what protein relieves stress in the helical DNA caused by the unwinding?
DNA gyrase (DNA topoisomerase 2)
____ copies of the DnaA protein bind to oriC
multiple
what two proteins are responsible for bringing in the DnaB protein?
DnaC and DnaT
DnaB subunits come in the form of a ____
hexomere
DnaB subunits come in the form of a ____
hexamer
SSB subunits come in the form of _____
tetromers
SSB subunits come in the form of _____
tetramers
what are the two enzymes responsible for DNA unwinding during the elongation phase?
DNA helicase (DnaB), gyrase (topoisomerase 2)
what enzyme is responsible for synthesizing an RNA primer that is needed for DNA replication in the elongation phase?
primase
what enzyme is used in the elongation phase to synthesize the new DNA strands?
DNA polymerase 3 holoenzyme
what subunit of DNA polymerase 3 acts as a “sliding clamp” that hold the DNA polymerase onto the DNA strand?
beta
what second enzyme is associated with DNA polymerase 1?
DNA ligase
what enzyme during elongation is responsible for binding to the region of the primers that will begin the Okazaki fragment to cut it out and replace it with a patch of DNA?
DNA polymerase 1
how many subunits are a part of DNA polymerase 3 holoenzyme?
10
what part of DNA polymerase 3 holoenzyme is involved in polymerase activity of linking new nucleotides to the end of the growing chain via covalent linkages?
alpha
what part of DNA polymerase 3 holoenzyme has 3’ to 5’ proofreading activity?
episilon
what part of DNA polymerase 3 holoenzyme stimulates the proofreading activity?
theta (O)
in DNA polymerase 3 holoenzyme, how many core units of A,E,T are present?
2