DNA nucleic acid Flashcards
3 models of dna replication
conservative model
semi conservative replication
fragmentary replication
semi conservative
each dna molecule contains one original print strand and one new strand half dna kept in tact
conservative model
one dna molecule has two original parent dna strands the other molecules has two new strands
fragmentary replication
all dna strands are made up of a mixture of original parent dna nucleotides and new nucleotides
meselson -stahl experiment
1)bacteria incorporate elements from their environment to make their dna
2)if they are grown in n15 their dna will be more dense
3)if they are grown in n14 their dna will be less dense
4)if the dna from bacteria is extracted and centrifuged the dna moved down the test tube according to its density .
do not want n14 and n 15 to mix
meselson -stahl
1)bacteria were originally grown in n 15 medium
2)making their dna more dense
3)they were then transferred to n14 media
4)any new dna replication would use lighter nucleotides
results of meselson -stahl
-only semi-conservative replication
-where replication produced dna molecules made of one original and one newly synthesis strand
-could explain the observed results in the first and second replicationmeseslo
what happens in semi conservative
1) dna helices hydrolyses dna molecule and breaks down hydrogen bonds in the nucleotides aka unwinds the dna
2)two strands are operated
dna polymerase joins the dna nucleotides together and forms a polymer of dna
3) 3 nucleotides pair up and dna polyemrsae joins via condensation reaction forming phosphodiester bonds
4)occurs along whole dna molecule producing two new dna molecules
5)each one original strand and one new strand
ATP
Universal currency for energy
usable amount of energy for biological rpocessesn
metbloci processes
provides the enrgy to build macromolecules from their basic units
movement
provides the energy to change the shape of carrier protein in plasma membrna es so that molecules can be transported against their conc gradient
secretion
used to form lysosome
atp
-universal currency for energy
-usable energy for biological processes
glucose how many atp
30
what can tap be used for
metabolic processes
movement
secretion
active transport
activation of molecules