DNA nucleic acid Flashcards

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1
Q

3 models of dna replication

A

conservative model
semi conservative replication
fragmentary replication

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2
Q

semi conservative

A

each dna molecule contains one original print strand and one new strand half dna kept in tact

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3
Q

conservative model

A

one dna molecule has two original parent dna strands the other molecules has two new strands

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4
Q

fragmentary replication

A

all dna strands are made up of a mixture of original parent dna nucleotides and new nucleotides

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5
Q

meselson -stahl experiment

A

1)bacteria incorporate elements from their environment to make their dna
2)if they are grown in n15 their dna will be more dense
3)if they are grown in n14 their dna will be less dense
4)if the dna from bacteria is extracted and centrifuged the dna moved down the test tube according to its density .

do not want n14 and n 15 to mix

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6
Q

meselson -stahl

A

1)bacteria were originally grown in n 15 medium
2)making their dna more dense
3)they were then transferred to n14 media
4)any new dna replication would use lighter nucleotides

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7
Q

results of meselson -stahl

A

-only semi-conservative replication
-where replication produced dna molecules made of one original and one newly synthesis strand
-could explain the observed results in the first and second replicationmeseslo

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8
Q

what happens in semi conservative

A

1) dna helices hydrolyses dna molecule and breaks down hydrogen bonds in the nucleotides aka unwinds the dna
2)two strands are operated
dna polymerase joins the dna nucleotides together and forms a polymer of dna
3) 3 nucleotides pair up and dna polyemrsae joins via condensation reaction forming phosphodiester bonds
4)occurs along whole dna molecule producing two new dna molecules
5)each one original strand and one new strand

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9
Q

ATP

A

Universal currency for energy
usable amount of energy for biological rpocessesn

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10
Q

metbloci processes

A

provides the enrgy to build macromolecules from their basic units

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11
Q

movement

A

provides the energy to change the shape of carrier protein in plasma membrna es so that molecules can be transported against their conc gradient

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12
Q

secretion

A

used to form lysosome

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13
Q

atp

A

-universal currency for energy
-usable energy for biological processes

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14
Q

glucose how many atp

A

30

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15
Q

what can tap be used for

A

metabolic processes
movement
secretion
active transport
activation of molecules

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16
Q

metabolic processes

A

provides the energy to build macromolecules from their basic units

17
Q

movement

A

provides the energy for muscle contraction energy is needed for the filaments to slide over each other

18
Q

active transport

A

provides the energy to change the shape of carrier proteins in the plasma membrane so that molecules can be transported against their concentration gradient

19
Q

secretion

A

used to form lysosomes
an enzyme inside cells to degrade molecules

20
Q

activation of molecules

A

the inorganic phosphate released can phosphorylate other compounds lowering the activation energy

21
Q

Pi

A

inorganic phhophate- doesn’t contain carbon

22
Q

hydrolysis of atp

A

ATP+water-> ADP +Pi+energy
exogonic reaction- release of all energy rather than just heat energy

happens immediately whenever energy is needed

23
Q

what is hydrolysis

A

the break down using a water molecule

24
Q

synthesis of atp

A

ADP+Pi -> atp+water
atp synthase is the enzyme responsible for this
endogenic reaction